Krioprotekcija hematopoetskih matičnih stanica

master's thesis

• Title (english): Cryopreservation of hematopoietic stem cells
• Author: Antonia Šoić
• Mentor: Sanja Dabelić (mentor)
• Committee member: Sanja Dabelić (predsjednik povjerenstva)
• Committee member: Sandra Šupraha-Goreta (član povjerenstva)
• Committee member: Domagoj Kifer (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2023-09-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Transplantacija hematopoetskih matičnih stanica koristi se u liječenju širokog raspona malignih
bolesti hematopoeze, ali i raznih nemalignih bolesti. Stanična terapija često zahtijeva stabilnu
pohranu stanica tijekom dugog razdoblja. Krioprotekcija se definira kao dugotrajno čuvanje živih
stanica i tkiva na ekstremno niskim temperaturama pri kojoj se stanični procesi privremeno
zaustavljaju. Osnovno načelo uspješne krioprotekcije stanica je sprječavanje stvaranja
unutarstaničnih i izvanstaničnih kristala leda tijekom zamrzavanja, jer je to primarni uzrok oštećenja
stanica. Kako bi se izbjegli nepovoljni učinci zamrzavanja, potrebno je koristiti krioprotektante koji
stupaju u interakciju s molekulama vode i snizuju točku ledišta vode. Dimetilsulfoksid (DMSO) je
daleko najčešće korišteni krioprotektant, unatoč njegovoj citotoksičnosti i razvojem nuspojava kod
pacijenata nakon infuzije staničnih proizvoda. Kako bi se smanjile neželjene reakcije povezane s
DMSO-om, istraživane su razne metode uklanjanja DMSO-a prije infuzije, kao i pronalazak novih
netoksičnih krioprotektanta. Osim dodavanja krioprotektanata stanicama, za uspješnu krioprotekciju
potrebno je regulirati i niz drugih parametara, kao što su koncentracija stanica, brzina zamrzavnja,
temperatura pohrane i brzina odmrzavanja. Na kraju, važno je procijeniti vijabilnost i funkcionalnost
odmrznutih stanica kako bi se provjerila kvaliteta proizvoda i procijenila uspješnost usađivanja.

Abstract (english)

Hematopoietic stem cells transplantation is used in the treatment of a wide range of malignant
diseases of hematopoiesis, as well as various non-malignant diseases. Cell therapy often requires
stable storage of cells over a long period. Cryoprotection is defined as long-term preservation of
living cells and tissues at extremely low temperatures, during which cellular processes are
temporarily stopped. The basic principle of successful cryoprotection of cells is to prevent the
formation of intracellular and extracellular ice crystals during freezing, as this is the primary cause
of cell damage. In order to avoid the unfavorable effects of freezing, it is necessary to use
cryoprotectants which interact with water molecules and lower the freezing point of water.
Dimethylsulfoxide (DMSO) is by far the most commonly used cryoprotectant, despite its
cytotoxicity and the development of side effects in patients after infusion of cell products. In order
to reduce the adverse reactions associated with DMSO, various methods of removing DMSO prior
to infusion have been investigated, as well as the discovery of new non-toxic cryoprotectants. In
addition to adding cryoprotectants to cells, for successful cryoprotection it is necessary to regulate a
number of other parameters, such as cell concentration, freezing rate, storage temperature and
thawing rate. Finally, it is important to assess the viability and functionality of the thawed cells to
verify product quality and assess engraftment success.

• Keywords: hematopoetske matčne stanice; krioprotekcija stanica; dimetilsulfoksid; krioprotektanti bez DMSO-a
• Keywords (english): hematopoietic stem cells; cryopreservation of cells; dimethyl sulfoxide; DMSO-free cryoprotectant
• Language: croatian
• URN:NBN: urn:nbn:hr:163:245807
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-03-05
• Created on: 2024-01-04 14:46:37