Optimizacija postupka in vitro oslobađanja ciprofloksacina iz vezikularnih fosfolipidnih gelova

master's thesis

• Title (english): Optimization of a procedure for performing in vitro release of ciprofloxacin from vesicular phospholipid gels
• Author: Ena Jurina
• Mentor: Željka Vanić (mentor)
• Committee member: Željka Vanić (predsjednik povjerenstva)
• Committee member: Zora Rukavina (član povjerenstva)
• Committee member: Lovorka Vujić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2023-06-13, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Formulacije vezikularnih fosfolipidnih gelova (VFG), koje su se međusobno razlikovale
fosfolipidnim sastavom, prisustvom polimera (kitozan) i suotapala (propilenglikol), pripremljene
su hidratizacijom fosfolipida u vodenoj otopini ciprofloksacina uz primjenu visokotlačnog
homogenizatora. Srednji promjer liposoma unutar VFG-a kretao se od 126 do 176 nm, uz indeks
polidisperznosti manji od 0,43 te zeta potencijal od +25 do +33 mV, dok je uspješnost uklapanja
iznosila od 25 do 31%. Ispitivanje in vitro oslobađanja ciprofloksacina iz VFG-a provedena je
primjenom metode membranske difuzije tijekom 30 sati. Ispitivanja su provedena u vodi te u puferu
pH 7,4 kao medijima za oslobađanje. Kao kontrola služila je vodena otopina ciprofloksacina.
Optimizacija postupka provedena je na formulaciji koja je sadržavala samo SPC 100, te su pod istim
uvjetima provedena ispitivanja s formulacijama koje su uz SPC 100 sadržavale SLPC-80 i
propilenglikol (elastični dvoslojevi), odnosno SPC 100 i kitozan. Oslobađanje je bilo najbrže iz
formulacije karakterizirane elastičnim fosfolipidnim dvoslojevima, dok su druge dvije formulacije,
kojima se fosfolipidni dvosloj sastojao od SPC 100, odnosno SPC 100 i kitozana, osiguravale sporije
oslobađanje lijeka (neovisno o korištenom dijalizacijskom mediju).

Abstract (english)

Vesicular phospholipid gels (VPG), differing in phospholipid composition, presence of chitosan
and/or propylene glycol, were prepared by hydration of phospholipids in an aqueous solution of
ciprofloxacin, followed by homogenization in a high-pressure homogenizer. The mean diameters of
liposomes inside the VPGs ranged from 126 to 176 nm, with a polydispersity indexes less than 0.43,
and zeta potentials from +25 to +33 mV, while the encapsulation efficiency amounted from 25 to
31%. A membrane diffusion method was applied to test in vitro release of ciprofloxacin from the
different VPGs in water and buffer, pH 7.4 during 30 hours. An aqueous solution of ciprofloxacin
served as a control. Optimization of in vitro release conditions was conducted on VPG composed of
only SPC 100. The optimized conditions were applied for performing in vitro release studies from
VPGs which, in addition to SPC 100, contained SLPC 80 and propylene glycol (elastic bilayers), or
SPC 100 and chitosan. Somewhat faster release of ciprofloxacin was achieved from the VPG
characterized by elastic bilayers, while the other two formulations of VPGs exhibited slower drug
release, regardless of the dialysis medium used.

• Keywords: vezikularni fosfolipidni gel; ciprofloksacin; ispitivanje in vitro oslobađanja lijeka; dermalna primjena
• Keywords (english): vesicular phospholipid gel; ciprofloxacin; in vitro drug release; dermal drug delivery
• Language: croatian
• URN:NBN: urn:nbn:hr:163:342451
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-06-13
• Created on: 2024-01-04 15:56:49