Bioraznolikost plijesni u vlažnom stambenom prostoru

master's thesis

• Title (english): Biodiversity of mold in a damp dwelling
• Author: Lada Sviben
• Mentor: Daniela Jakšić (mentor)
• Committee member: Daniela Jakšić (predsjednik povjerenstva)
• Committee member: Maja Šegvić Klarić (član povjerenstva)
• Committee member: Manuela Zadravec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology); Zagreb
• Defense date and country: 2023-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Stambeni prostori pogođeni vlagom pružaju idealne uvjete plijesnima za razmnožavanje. Povišena koncentracija određenih vrsta plijesni može uzrokovati ponajprije respiratorne simptome i bolesti u ljudi koji u tim prostorima provode duže vrijeme. Izolati plijesni iz zraka prikupljenih u stana pogođenog vlagom te iz vanjskog zraka (kontrolni uzorci) identificirani su do razine vrste na temelju molekularnog markera ITS za gljive te usporedbom nukleotidnih slijedova pojedinog izolata s podacima u javno dostupnim bazama podataka (NCBI, ISHAM). Veću bioraznolikost pokazali su izolati iz unutarnjeg prostora gdje su identificirane vrste: Aspergillus aureolatus (N=1), A. jensenii (N=1), A. purpureocrustaceus (N=11), A. sulphureoviridis (N=2), A. welwitschiae/A. niger (N=1), Cladosporium cycadicola (N=2), Penicillium robsamsonii (N=5), P. robsamsonii/P.hordei (N=1), P. rubens (N=1), P. speluncae (N=4), Stachybotrys limonispora/S. chartarum (N=1), Talaromyces wortmanii (N=1), Trametes versicolor (N=1) i Verruciconidia persicana (N=1). Vrste nađene samo u vanjskom zraku bile su Beauveria pseudobassiana (N=2) i Samsoniella hepiali (N=1), dok su vrste Ascochyta phacae (N=2), P. bialowiezense (N=5) i P. fimorum (N=10) identificirane u uzorcima unutarnjeg i vanjskog zraka. Dobiveni rezultati upućuju na razlike u identifikaciji obzirom na korištenu bazu podataka NCBI ili ISHAM te potrebu za ažuriranjem baze ISHAM nukleotidnim slijedovima novoopisanih vrsta roda Penicillium i Aspergillus. Upotreba sekundarnih molekularnih i/ili kemijskih markera omogućava preciznu identifikaciju plijesni do razine vrste zbog preklapanja u nukleotidnim slijedovima u regiji ITS među srodnim vrstama plijesni.

Abstract (english)

Residential spaces affected by moisture provide ideal conditions for mold proliferation. Elevated concentrations of certain mold species can primarily cause respiratory symptoms and illnesses in individuals who spend extended periods of time in these areas. Mold isolates were recovered from indoor air sampled in moisture-affected homes and from outdoor air (control samples). Identification to the species level was conducted based on the ITS molecular marker for fungi by comparing nucleotide sequences of individual isolates with data in publicly available databases (NCBI, ISHAM). Greater biodiversity was observed among the indoor air isolates, and the following species were identified: Aspergillus aureolatus (N=1), A. jensenii (N=1), A. purpureocrustaceus (N=11), A. sulphureoviridis (N=2), A. welwitschiae/A. niger (N=1), Cladosporium cycadicola (N=2), Penicillium robsamsonii (N=5), P. robsamsonii/P.hordei (N=1), P. rubens (N=1), P. speluncae (N=4), Stachybotrys limonispora/S. chartarum (N=1), Talaromyces wortmanii (N=1), Trametes versicolor (N=1), and Verruciconidia persicana (N=1). The outdoor air isolates were identified to Beauveria pseudobassiana (N=2) and Samsoniella hepiali (N=1), while the species Ascochyta phacae (N=2), P. bialowiezense (N=5), and P. fimorum (N=10) were identified in both indoor and outdoor air samples. The results obtained indicate differences in identification depending on the NCBI or ISHAM database used and suggest updating the ISHAM database with nucleotide sequences of newly described species of the genus Penicillium and Aspergillus. The use of secondary molecular and/or chemical markers allows for precise mold identification at the species level due to overlap in nucleotide sequences in the ITS region among closely related mold species.

• Keywords: baza ISHAM; baza NCBI; vlaga; plijesni; Aspergillus; Penicillium; Stachybotrys
• Keywords (english): NCBI database; ISHAM database; dampness; mould; Aspergillus; Penicillium; Stachybotrys
• Language: croatian
• URN:NBN: urn:nbn:hr:163:709698
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-01-23 11:17:27