Kemijska karakterizacija i biološki učinci polifenola u vinu

doctoral thesis

• Title (english): Chemical characterization and biological effects of polyphenols in wines
• Author: Matija Kropek
• Mentor: Sanda Vladimir-Knežević (mentor)
• Committee member: Biljana Blažeković (predsjednik povjerenstva)
• Committee member: Željan Maleš (član povjerenstva)
• Committee member: Renata Jurišić Grubešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2023-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy
• Universal decimal classification (UDC): 615 - Pharmacology. Therapeutics. Toxicology

Abstract

Vina sadrže različite biološki aktivne polifenolne spojeve za koje je utvrđeno da imaju brojne pozitivne učinke na zdravlje.
Polifenolni profil vina često se koristi za procjenu njihove kvalitete i autentičnosti. U okviru doktorskog rada provedena je
usporedna kemijska karakterizacija polifenola u dvadeset i pet komercijalnih crnih i bijelih vina proizvedenih u različitim
hrvatskim vinogradarskim regijama od osam crnih i devet bijelih sorti grožđa. U tu je svrhu razvijena i validirana metoda
visokoučinske tekućinske kromatografije (HPLC-DAD) s direktnim injektiranjem vina. Analizirane su dvadeset i četiri
polifenolne sastavnice vina te razvrstane u pet skupina na temelju njihove strukture: fenolne kiseline, flavan-3-ole, antocijane,
flavonoide i stilbene. Crna vina sadržavala su značajno veće koncentracije polifenola od bijelih vina te je kod njih uočena
velika varijabilnost u sastavu. Specifičnim polifenolnim sastavom i sadržajem posebice su se istaknula vina proizvedena od
autohtonih hrvatskih sorti plavac mali i babić. Primjenom multivarijatne analize ustanovljene su sličnosti i razlike u
polifenolnim profilima hrvatskih monosortnih vina te detektirani važni diferencijatori. Glavni predstavnici polifenolnih
sastavnica koji su bili značajno zastupljeni u istraživanim vinima pokazali su snažno antioksidacijsko djelovanje te sposobnost
inhibicije aktivnosti enzima Src-tirozin-kinaze, α-glukozidaze i acetilkolinesteraze. Provedeno istraživanje pružilo je nove
spoznaje o polifenolnim profilima hrvatskih vina, dajući doprinos njihovoj sortnoj diferencijaciji, autentifikaciji i kontroli
kvalitete. Ustanovljeno je da su analizirana crna vina, prvenstveno ona dobivena iz hrvatskih autohtonih crnih sorti grožđa,
vrijedan izvor polifenola sa snažnim antioksidacijskim svojstvima i sposobnošću inhibicije enzima koji predstavljaju važne
terapijske mete u prevenciji i liječenju nekih kroničnih bolesti povezanih sa starenjem.

Abstract (english)

Wines contain various biologically active polyphenolic compounds that are belived to have numerous beneficial effects on
human health. The polyphenolic profile of wines is often used to evaluate their quality and authenticity. In this doctoral thesis, a comparative chemical characterization of polyphenols was performed in twenty-five commercial red and white wines produced in different Croatian wine-growing regions from eight black and nine white grape varieties. For this purpose, a method of high-performance liquid chromatography (HPLC-DAD) with direct injection of wine was developed and validated. Twentyfour polyphenolic components of the wines were studied and classified into five groups based on their structure: phenolic acids, flavan-3-ols, anthocyanins, flavonoids and stilbenes. Black wines contained significantly higher concentrations of polyphenols than white wines, and a great variability in their composition was found. Wines produced from the native Croatian varieties Plavac mali and Babić were characterized by their specific polyphenolic composition and content. Multivariate analysis was used to determine similarities and differences in the polyphenolic profiles of Croatian monovarietal wines and to identify important differentiators. The main representatives of polyphenolic components, which were significantly present in the studied wines, showed strong antioxidant activity and the ability to inhibit the activity of Src tyrosine kinase, α-glucosidase and acetylcholinesterase enzymes. The conducted research provided new insights into the polyphenolic profiles of Croatian wines, thus contributing to their varietal differentiation, authentication and quality control. It was found that the analyzed red wines, especially those obtained from Croatian autochthonous red grape varieties, are a valuable source of polyphenols with strong antioxidant properties and the ability to inhibit enzymes that are important therapeutic targets in the prevention and treatment of some chronic diseases associated with aging.

• Keywords: hrvatska vina; polifenoli; HPLC-DAD; PCA; antioksidacijsko djelovanje; inhibicija enzima; Src-tirozinkinaza; α-glukozidaza; acetilkolinesteraza
• Keywords (english): Croatian wines; polyphenols; HPLC-DAD; PCA; antioxidant activity; enzymes inhibition; Src tyrosine kinase; α-glucosidase; acetylcholinesterase
• Language: croatian
• URN:NBN: urn:nbn:hr:163:450726
• Promotion: 2023
• Study programme: Title: Pharmacy and biochemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor znanosti (doktor znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-01-11
• Created on: 2024-01-23 12:37:24