Učinak ekstrakta lista masline na metaboličku aktivnost stanica humanih staničnih linija melanoma A375 i A375M

master's thesis

• Title (english): The effect of olive leaf extract on the metabolic activity of human melanoma cell lines A375 and A375M
• Author: Ivona Brajdić
• Mentor: Sanja Dabelić (mentor)
• Committee member: Sanja Dabelić (predsjednik povjerenstva)
• Committee member: Domagoj Kifer (član povjerenstva)
• Committee member: Sandra Šupraha-Goreta (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2023-10-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Zbog nedovoljno efikasnih i opasnih terapijskih opcija za liječenje melanoma, potrebno je istražiti potencijalne alternativne terapijske pristupe. Cilj je ovoga rada ispitati kako primjena ekstrakta lista masline (O. europaea) sorte 'Rosinjola' (sinonim 'Rošinjola', 'Rovinježa') i 'Istarska bjelica' (sinonim 'Bianchera') utječe na metabolizam i preživljavanje humanih stanica staničnih linija melanoma A375, melanoma s visokom sposobnošću metastaziranja A375M i spontano imortaliziranih keratinocita HaCaT. Za određivanje metaboličke aktivnosti stanica nakon 48-satne izloženosti različitim koncentracijama ekstrakta lista masline korišten je kolorimetrijski MTS test CellTiter 96®AQueous One Solution Cell Proliferation Assay. Rezultati su obrađeni prikladnim statističkim metodama pomoću programa GraphPad Prism, verzija 10.0.2 te je izračunata vrijednost IC50. Utvrđeno je da jačina i obrazac učinka ovisi o tipu stanične linije i primijenjenoj koncentraciji ekstrakta. Najintenzivnije djelovanje uočeno je kod stanične linije A375, zatim kod HaCaT, a najslabije kod stanične linije A375M. Daljnja su istraživanja potrebna kako bi se utvrdilo koje su sastavnice ekstrakta odgovorne za uočeni učinak i kojim se molekularnim mehanizmima taj učinak ostvaruje.

Abstract (english)

Due to insufficiently effective and dangerous therapeutic options for the treatment of melanoma, it is necessary to explore potential alternative therapeutic approaches. The aim of this thesis is to examine how the application of olive leaf extract (O. europaea) of the variety 'Rosinjola' (synonymous to 'Rošinjola', 'Rovinježa') and 'Istarska bjelica' (synonymous to 'Bianchera') affects the metabolism and survival of human melanoma cell line A375, highly metastatic human melanoma cell line A375M and spontaneously immortalized keratinocytes cell line HaCaT. For determining the metabolic activity of cells after a 48-hour exposure to different concentrations of olive leaf extract, the colorimetric MTS test CellTiter 96®AQueous One Solution Cell Proliferation Assay was used. The results were analyzed using the appropriate statistical methods within the GraphPad Prism program, version 10.0.2, and the IC50 value was calculated. The strength and pattern of the effect was found to depend on the type of cell line used and the applied concentration of the extract. The most intense effect was observed with the A375 cell line, followed by HaCaT, and the weakest with the A375M cell line. Further research is needed to determine which components of the extract are responsible for the observed effect and by which molecular mechanisms is this effect achieved.

• Keywords: ekstrakt lista masline; melanom; keratinociti; kolorimetrijski MTS test
• Keywords (english): olive leaf extract; melanoma; keratinocytes; colorimetric MTS test
• Language: croatian
• URN:NBN: urn:nbn:hr:163:015822
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-04-30
• Created on: 2024-01-25 13:43:55