Kritička procjena energetski disperzivne rendgenske fluorescencijske spektrometrije za multielementarnu analizu kave

master's thesis

• Title (english): Critical evaluation of energy dispersive X-ray fluorescence spectrometry for multielemental analysis of coffee
• Author: Ana Todorić
• Mentor: Jasna Jablan (mentor)
• Committee member: Jasna Jablan (predsjednik povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2024-03-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Kava je jedan od najpopularnijih napitaka diljem svijeta stoga je ispitivanje sastava kave od iznimne
važnosti kako za proizvođače tako i za potrošače. Minerali zastupljeni u kavi su, osim za kvalitetu
krajnjeg proizvoda, bitni sa zdravstvenog gledišta jer neki od njih djeluju kao antioksidansi,
sudjeluju u biološkim procesima u organizmu, a određeni posjeduju i toksične učinke. Cilj ovog rada
bio je kritički procijeniti EDXRF tehniku za multielementarnu analizu uzoraka čiste kave, instant
kave i kave s dodacima koristeći rezultate dobivene ICP-OES tehnikom kao referentne vrijednosti.
Ispitani su najbolji način pripreme uzorka za EDXRF analizu, najprikladniji uvjeti mjerenja te
najtočniji način kvantifikacije uzoraka. Za provjeru kvalitete rezultata dobivenih primjenom ICPOES
tehnike analizirana su tri organska referentna materijala te uspoređena sa spomenutim
rezultatima. Nadalje, različite kemometrijske tehnike kao što su PCA i PLS-DA primijenjene su na
dobivene podatke kako bi se istražio njihov potencijal za klasifikaciju različitih vrsta kava. Dobiveni
rezultati u ovom radu pokazali su da mogu biti korisni za uspostavljanje pouzdane jednostavne
metode za multielementarnu analizu različitih uzoraka kave pomoću EDXRF-a, kao i za pružanje
dobre osnove za daljnju primjenu EDXRF spektara kao otiska uzorka u drugim studijama sljedivosti
i/ili provjere autentičnosti prehrambenih proizvoda u budućnosti.

Abstract (english)

Coffee is one of the most popular beverages around the world, which is why studying the
composition of coffee is extremely important for both producers and consumers. The minerals
contained in coffee are, not only important for the quality of the final product, but also for health as
some of them act as antioxidants, and are involved in biological processes in the body, while others
have toxic effects. The aim of this work was to critically evaluate the EDXRF technique for the
multielemental analysis of samples of pure coffee, instant coffee and coffee with additives using the
results obtained with the ICP-OES technique as reference values. The best method of sample
preparation for EDXRF analysis, the most suitable measurement conditions and the most accurate
method for quantifying the samples were examined. In order to verify the quality of the results
obtained with the ICP-OES technique, three organic reference materials were analyzed and
compared with the aforementioned results. Finally, different chemometric methods such as PCA and
PLS-DA were applied to the data obtained to investigate their potential for the classification of
different coffee varieties. It is concluded that the results obtained in this work can be useful to
establish a reliable simple method for the multielemental analysis of different coffee samples by
EDXRF and provide a good basis for further application of EDXRF spectra as sample fingerprint ing
in other traceability and/or authentication studies of food in the future.

• Keywords: minerali; kava; energetski disperzivna rendgenska fluorescencijska spektrometrija
• Keywords (english): minerals; coffee; energy dispersive X-ray fluorescence spectrometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:691537
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-05-23 13:54:40