Kontrola kakvoće biljne droge Verbasci flos

master's thesis

• Title (english): Quality control of Verbasci flos
• Author: Doroteja Palčić
• Mentor: Maja Bival Štefan (mentor)
• Committee member: Maja Bival Štefan (predsjednik povjerenstva)
• Committee member: Marijana Zovko Končić (član povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2024-03-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovog diplomskog rada uspoređena su dva uzorka droge Verbasci flos sakupljena na dvije različite lokacije te je provedena kontrola kakvoće tih uzoraka. Određivanjem broj bubrenja i gubitka sušenjem ustanovljeno je da uzorci odgovaraju farmakopejskim zahtjevima, dok je u uzorku 2 pronađena nešto veća količina fragmenta čaške i ostalih stranih tvari. Mikroskopskim pregledom pronađeni su svi dijelovi koje Farmakopeja propisuje. Tankoslojnom kromatografijom (TLC) dokazana je prisutnost flavonoida i fenolnih kiselina koje su potom određene UV-Vis spektrofotometrijskom metodom te je sadržaj iznosio 0,053-0,075% i 0,08- 0,24%. Također određen je i sadržaj trjeslovina koji je iznosio 0,172-0,24%.

Abstract (english)

As part of this thesis, two types of mullein flower from two different locations were compared and the quality control of these samples was carried out. By determining the swelling index and loss on drying, it was found that the samples correspond to pharmacopoeial requirements, while in the sample 2 there was slightly larger amount of fragments of the calyx, and other foreign substances was found. By microscopic examination we found all the parts prescribed by the Pharmacopoeia. Thin-layer chromatography (TLC) showed the presence of flavonoids and phenolic acids, which were then quantified by UV-vis spectrometric method, with the content ranging from 0.053% to 0.075% and 0.08% to 0.24%. The content of tannins was also determined, ranging from 0.172% to 0.24%.

• Keywords: divizma; Verbascum phlomoides; fenolne kiseline; flavonoidi
• Keywords (english): mullein; Verbascum phlomoides; phenolic acid; flavonoids
• Language: croatian
• URN:NBN: urn:nbn:hr:163:846262
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-03-25
• Created on: 2024-05-23 14:20:38