Optimizacija ultrazvučne ekstrakcije polifenolnih spojeva iz vrste Satureja montana L.

master's thesis

• Title (english): Optimisation of ultrasound extraction of polyphenols from species Satureja montana L.
• Author: Iva Petrović
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2024-03-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

S. montana L. aromatična je biljaka bogata bioaktivnim fenolnim spojevima. U ovom istraživanju
spektrofotometrijski je ispitivan sadržaj ukupnih polifenolnih spojeva, fenolnih kiselina i flavonoida
te sadržaj luteolin-7-O-glikozida upotrebom HPLC-a. Korištena je ekološki prihvatljiva metoda
ultrazvučne ekstrakcije te su primjenjivani različiti ekstrakcijski uvjeti. Cilj je bio pronaći uvjete
koji daju najučinkovitiju ekstrakciju s najvećim prinosom fenolnih spojeva. Pokazana je efikasnost
prisustva HP-β-CD, kao sredstva koje omogućuje inkapsulaciju polifenola, te kombinacije glicerola
i vode kao otapala. Najveći prinos ukupnih polifenola i fenolnih kiselina dao je pripravak s uvjetima
70 % GL, 0,2 mmol HP-β-CD, pri 45 °C, 25 min i USP od 432 W. Ekstrakciji flavonoida najviše su
pogodovali uvjeti 35 % GL, 0,2 mmol HP-β-CD, 15 min na 70 °C s USP od 720 W, a LG uvjeti 35
% GL, 0,2 mmol HP-β-CD, 20 °C, 15 min s USP od 720 W.

Abstract (english)

S. montana L. is an aromatic species rich in bioactive phenolics. In this work, spectrophotometric
analysis of total polyphenols, phenolic acids and flavonoids in the extract was conducted, as well as
content quantification of luteolin-7-O-glicoside using HPLC. Environmentally friendly ultrasound
extraction method was used, and different extraction conditions were applied. The purpose was to
find conditions giving the most effective yield of measured substances. The results have shown the
efficiency of having HP-β-CD present, as an encapsulating agent, as well as glycerol – water mixture
as a solvent. The biggest yield of total polyphenols and phenolic acids was shown in extraction
conditions of 70 % GL, 0,2 mmol HP-β-CD, 45 °C, 25 min and USP of 432 W. To the flavonoid
extraction favoured the most conditions of 35 % GL, 0,2 mmol HP-β-CD, 15 min on 70 °C with
USP of 720 W, and for LG conditions of 35 % GL, 0,2 mmol HP-β-CD, 20 °C, 15 min and USP of
720 W.

• Keywords: Satureja montana; polifenoli; glicerol; (2-hidroksipropil)-β-ciklodekstrin; luteolin-7-O-glikozid
• Keywords (english): Satureja montana; polyphenols; glycerol; (2-hydroxypropyl)-β-cyclodextrin; luteolin-7-O-glikoside
• Language: croatian
• URN:NBN: urn:nbn:hr:163:897178
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-03-27
• Created on: 2024-05-23 14:32:24