Fitokemijska karakterizacija ekstrakata biljne droge Melissae folium

master's thesis

• Title (english): Phytochemical characterization of the Melissae folium extracts
• Author: Mario Bahlen
• Mentor: Biljana Blažeković (mentor)
• Committee member: Biljana Blažeković (predsjednik povjerenstva)
• Committee member: Valerija Vujčić Bok (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2024-04-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Matičnjakov list (Melissae folium) je ljekovita biljna droga koja se temeljem dugogodišnjeiskustvene primjene preporuča kod blagih simptoma stresa, kao pomoć pri spavanju i protiv blažihprobavnih tegoba. Povoljan utjecaj na ljudsko zdravlje pripisuje se sadržaju polifenolnih iterpenskih sastavnica te je stoga važno odrediti njihov sadržaj i sastav u ekstraktima spotencijalnom primjenom u prevenciji i fitoterapiji.
U okviru ovog diplomskog rada provedena je fitokemijska karakterizacija različitih ekstrakatamatičnjakovog lista. U tu su svrhu u vodenim i vodenoetanolnim ekstraktimaspektrofotometrijskim metodama određeni sadržaji ukupnih flavonoida i fenolnih kiselina te jeHPLC-DAD metodom određen sadržaj ružmarinske kiseline kao najzastupljenije polifenolnebioaktivne sastavnice. GC-MS metodom provedena je analiza sastavnica eteričnog uljamatičnjakovog lista. Dobiveni rezultati doprinose znanstvenim spoznajama o bioaktivnimsastavnicama i biomedicinskom potencijalu ekstrakata matičnjaka hrvatskog porijekla.

Abstract (english)

Lemon balm leaf (Melissae folium) is a herbal drug that is, due to experience with its longtraditional use, recommended as medicine for mild mental stress symptoms relief, to aid sleep andmild gastrointestinal complaints. Its beneficial effect on human health is related to the content ofpolyphenolic and terpene compounds and because of that it is important to determine their contentand composition in extracts with the potential of being used in prevention and phytotherapy.
In this thesis a phytochemical characterization of different lemon balm leaf extracts was done.Spectrophotometric methods were used to determine the content of total flavonoids and phenolicacids in aqueous and hydroalcoholic extracts and HPLC-DAD method was used to determine thecontent of rosmarinic acid as a main polyphenolic bioactive compund. GC-MS method was used toanalyse the compounds of the lemon balm leaf essential oil, The obtained results contribute to thescientific knowledges on the bioactive compounds and biomedicinal potential of the lemon balmextracts from Croatia.

• Keywords: matičnjak; matičnjakov list; Melissae folium; flavonoidi; fenolne kiseline; ružmarinska kiselina; eterično ulje; HPLC-DAD; GC-MS
• Keywords (english): lemon balm; lemon balm leaf; Melissae folium; flavonoids; phenolic acids; rosmarinic acid; essential oil; HPLC-DAD; GC-MS
• Language: croatian
• URN:NBN: urn:nbn:hr:163:397479
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-04-18
• Created on: 2024-06-13 12:10:06