Fitokemijski sastav i antioksidacijsko djelovanje ploda vrste Ribes uva-crispa L. (Grossulariaceae)

master's thesis

• Title (english): Phytochemical composition and antioxidant activity of fruit of Ribes uva-crispa L. (Grossulariaceae)
• Author: Marija Bakran
• Mentor: Maja Bival Štefan (mentor)
• Committee member: Maja Bival Štefan (predsjednik povjerenstva)
• Committee member: Biljana Blažeković (član povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2024-04-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Ovim diplomskim radom analiziran je fitokemijski sastav te je ispitano antioksidacijsko djelovanje plodova vrste Ribes uva-crispa L. Identifikacija flavonoida (rutina, kvercitrina i izokvercitrina) i fenolnih kiselina (klorogenske kiseline) provedena je primjenom tankoslojne kromatografije. U vodenom i metanolnom ekstraktu plodova R. uva-crispa L. utvrđena je prisutnost rutina i izokvercitrina te klorogenske kiseline. Kvantifikacija fenolnih kiselina (izraženih kao klorogenska kiselina) i ukupnih polifenola (izraženih kao galna kiselina) provedena je spektrofotometrijskim metodama.
Sadržaj fenolnih kiselina, izražen kao klorogenska kiselina iznosio je 0,45 %, dok je udio ukupnih polifenola, izražen kao galna kiselina iznosio 2,5 %. Antioksidacijsko djelovanje ekstrakta ispitano je dvjema spektrofotometrijskim metodama: određivanjem sposobnosti hvatanja DPPH slobodnog radikala (IC50: 331,56 ± 15,90 μg/mL,) te određivanjem sposobnosti hvatanja NO radikala (IC50: 332,22 ± 11,22 μg/mL).
Ovaj rad potvrdio je fitoterapijski potencijal vrste Ribes uva-crispa L.

Abstract (english)

In this master's thesis the phytochemical composition and antioxidant activity of the fruits of Ribes uva-crispa L. were analyzed. The identification of flavonoids (rutin, quercitrin and isoquercitrin) and phenolic acids (chlorogenic acid) was conducted using thin-layer chromatography. The presence of rutin, isoquercitrin and chlorogenic acid was determined in the water and methanol extracts of R. uva-crispa L. fruits. The quantification of phenolic acids (expressed as chlorogenic acid) and total polyphenols (expressed as gallic acid) was performed using spectrophotometric methods. The content of phenolic acids, expressed as chlorogenic acid was 0,45 %, while the amount of total polyphenols, expressed as gallic acid was 2,5 %. The antioxidant activity of extracts was defined using two spectrophotometric methods: the determination of the DPPH free radical scavenging ability (IC50: 331,56 ± 15,90 μg/mL) and the determination of the NO radical scavenging ability (IC50: 332,22 ± 11,22 μg/mL).
This study confirmed the phytotherapeutic potential of Ribes uva-crispa L.

• Keywords: Ribes uva-crispa L.; flavonoidi; fenolne kiseline; antioksidacijsko djelovanje
• Keywords (english): Ribes uva-crispa L.; flavonoids; phenolic acids; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:163:326217
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-04-15
• Created on: 2024-06-13 12:16:59