Analiza rezultata određivanja Cu i Zn u uzorcima folikularne tekućine žena s dijagnozom idiopatske neplodnosti koje se liječe postupkom potpomognute oplodnje

master's thesis

• Title (english): Analysis of Cu and Zn determination results in follicular fluid samples from women diagnosed with idiopathic infertility undergoing assisted reproductive treatment
• Author: Lucija Sajko
• Mentor: Anita Somborac Bačura (mentor)
• Mentor: Ivana Zec (mentor)
• Committee member: Anita Somborac Bačura (predsjednik povjerenstva)
• Committee member: Marija Grdić Rajković (član povjerenstva)
• Committee member: Ivana Zec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2024-07-12, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Neplodnost je stanje koje karakterizira nemogućnost trudnoće nakon barem 12 mjeseci nezaštićenih spolnih odnosa, a pogađa
otprilike 15 % parova u svijetu. Unatoč brojnim poznatim uzrocima, u nekim slučajevima oni ipak nisu poznati, odnosno radi se o
idiopatskoj neplodnosti. U liječenju se najčešće koristi farmakoterapija u kombinaciji s postupcima potpomognute oplodnje.
Folikularna tekućina prirodno je okruženje oocite, a analiza elemenata te tekućine omogućava procjenu kvalitete jajne stanice i
sposobnosti oplodnje. U sastavu folikularne tekućine nalaze se i bakar i cink koji su, nakon željeza, najzastupljeniji elementi u tragu
u organizmu te imaju brojne funkcije. Njihova koncentracija u folikularnoj tekućini pozitivno korelira sa stopom oplodnje. Hipoteza
ovog istraživanja je da ovarijska stimulacija mijenja koncentraciju bakra i cinka u folikularnoj tekućini žena s dijagnozom
idiopatske neplodnosti u odnosu na modifikaciju prirodnog ciklusa. U istraživanje su bile uključene 52 pacijentice koje su se u
Kliničkom bolničkom centru Sestre milosrdnice liječile postupkom potpomognute oplodnje. 27 pacijentica podvrgnuto je postupku
u modificiranom prirodnom ciklusu, a kod preostalih 25 pacijentica provodila se ovarijska stimulacija. Atomskom apsorpcijskom
spektrometrijom u uzorcima folikularne tekućine određena je koncentracija bakra i cinka te su podatci obrađeni statističkim
programom MedCalc. Uočena je statistički značajna razlika u koncentraciji bakra i cinka u dvije skupine ispitanica te je zaključeno
da ovarijskom stimulacijom ciklusa dolazi do porasta koncentracije bakra i cinka u folikularnoj tekućini u odnosu na modificirani
prirodni ciklus. Povećane koncentracije bakra i cinka povezane su s boljom kvalitetom jajne stanice i većom stopom oplodnje, a
također i s većom aktivnošću superoksid-dismutaze budući da je uslijed stimulacije ciklusa povećan oksidacijski stres kod
pacijentica. Kako nisu poznati referentni intervali za bakar i cink u folikularnoj tekućini, potrebna su daljnja istraživanja u kojima bi
se ispitala optimalna razina tih mikroelemenata u folikularnoj tekućini te njihov utjecaj na jajnu stanicu i oplodnju.

Abstract (english)

Infertility is a condition characterized by the inability to conceive after at least 12 months of unprotected intercourse, affecting
approximately 15% of couples worldwide. Despite numerous known causes, in some cases, the causes remain unknown, resulting in
idiopathic infertility. The most commonly used treatments include pharmacotherapy in combination with assisted reproductive
techniques. Follicular fluid is the natural environment of the oocyte and analyzing the elements within this fluid enables the
assessment of oocyte quality and fertilization potential. Copper and zinc, the most abundant trace elements in the body after iron, are
present in the composition of follicular fluid and have numerous functions in the body. Their concentration in the follicular fluid
positively correlates with fertilization rates. The hypothesis of this study is that ovarian stimulation alters the concentration of copper
and zinc in the follicular fluid of women diagnosed with idiopathic infertility compared to the modification of the natural cycle. The
study included 52 patients undergoing assisted reproductive treatment in the Sestre milosrdnice University Hospital Center. 27
patients underwent the procedure in a modified natural cycle, while the remaining 25 patients underwent ovarian stimulation. The
concentrations of copper and zinc in the follicular fluid samples were determined using atomic absorption spectrometry and the data
were analyzed using the statistical program MedCalc. A statistically significant difference in the concentration of copper and zinc
was observed between the two groups of participants, leading to the conclusion that ovarian stimulation increases the concentration
of copper and zinc in follicular fluid compared to the modified natural cycle. Increased concentrations of copper and zinc are
associated with better oocyte quality and higher fertilization rates, as well as greater activity of superoxide dismutase due to increased
oxidative stress in patients undergoing cycle stimulation. Since reference intervals for copper and zinc in follicular fluid are not
known, further research is needed to determine the optimal levels of these trace elements in follicular fluid and their impact on oocyte
quality and fertilization.

• Keywords: neplodnost; folikularna tekućina; ovarijska stimulacija; modificirani prirodni ciklus; bakar; cink; atomska apsorpcijska spektrometrija
• Keywords (english): infertility; follicular fluid; ovarian stimulation; modified natural cycle; copper; zinc; atomic absorption spectrometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:861061
• Study programme: Title: Medical biochemistry - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar medicinske biokemije i laboratorijske medicine (sveučilišni magistar medicinske biokemije i laboratorijske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-01-12
• Created on: 2024-07-26 08:01:54