Primjena TXRF-Mo i EDXRF tehnike za elementarnu analizu proteina sirutke

master's thesis

• Title (english): Application of TXRF-Mo and EDXRF techniques for elemental analysis of whey proteins
• Author: Tena Videković
• Mentor: Jasna Jablan (mentor)
• Committee member: Jasna Jablan (predsjednik povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of analytical chemistry); Zagreb
• Defense date and country: 2024-06-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Proteini sirutke su praškasti oblik proteina dobivenih iz sirutke. Pokazuju brojne korisne učinke na ljudsko zdravlje: antikancerogeni, antioksidativni i antidijabetički učinak, kardioprotektivno i hipotenzivno djelovanje, pozitivno utječu na probavno zdravlje. Na tržištu su dostupni u obliku hidrolizata proteina sirutke, koncentrata proteina sirutke i izolata proteina sirutke. Danas su u širokoj upotrebi pogotovo u fitnes industriji te je zbog toga nužna kontrola elementarnog sastava kako bi se spriječio potencijalno negativan utjecaj na ljudsko zdravlje. Glavni cilj ovog istraživanja je odrediti sadržaj metala u različitim uzorcima proteina sirutke pomoću dvije analitičke tehnike (TXRF-Mo i EDXRF) kao i usporediti učinkovitost primijenjenih tehnika za određivanje sadržaja metala te njihova primjenjivost u kontroli kvalitete proizvoda. Uzorci su pripremljeni mikrovalnom digestijom za potrebe mjerenja TXRF-Mo dok su kod EDXRF tehnike uzorci izravno mjereni bez prethodne pripreme te je određen sadržaj sljedećih elemenata: željezo, bakar, cink, brom, rubidij i stroncij. Dobiveni rezultati pokazali su kako su obje tehnike prikladne za multielementarno određivanje metala te imaju potencijal za određivanje sadržaja broma u uzorcima. Uslijed primijenjenog načina pripreme uzoraka u ovom ispitivanju, EDXRF tehnika je ekološki prihvatljivija i više u skladu s načelima „zelene kemije“ dok je TXRF-Mo osjetljivija tehnika.

Abstract (english)

Whey proteins are a powdered form of protein obtained from whey. They have numerous positive effects on human health: anti-cancer, antioxidant and anti-diabetic effects, cardioprotective and blood pressure lowering effects, positive effects on digestive health. They are available on the market in the form of whey protein hydrolysates, whey protein concentrates and whey protein isolates. Nowadays, they are widely used, especially in the fitness industry, which makes it necessary to control their elemental composition to avoid their potentially negative effects on human health. The main objective of this study is to determine the metal content in different whey protein samples using two analytical techniques (TXRF-Mo and EDXRF) and to compare the effectiveness of the techniques used to determine the metal content and their usefulness in controlling product quality. The samples for the TXRF-Mo measurements were prepared by microwave digestion, while the samples for the EDXRF technique were measured directly without prior preparation. The following elements were determined: iron, copper, zinc, bromine, rubidium and strontium. The results obtained showed that both techniques are suitable for the multi-element determination of metals and have the potential to determine the bromine content in samples. Due to the method of sample preparation used in this test, the EDXRF method proved to be more environmentally friendly and more in line with "green chemistry" principles," while TXRF-Mo is a more sensitive method.

• Keywords: sirutka; proteini sirutke; multielementarna analiza; EDXRF; TXRF
• Keywords (english): whey; whey proteins; multielemental analysis; EDXRF; TXRF
• Language: croatian
• URN:NBN: urn:nbn:hr:163:350094
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-07-20
• Created on: 2024-07-26 10:57:14