Fosfolipidni nanosustavi s timolom: izrada, karakterizacija i određivanje in vitro antimikrobne aktivnosti

master's thesis

• Title (english): Phospholipid nanosystems with thymol: preparation, characterization and in vitro antimicrobial assessment
• Author: Magda Vodolšak
• Mentor: Željka Vanić (mentor)
• Committee member: Željka Vanić (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Laura Nižić Nodilo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2024-07-04, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE

Abstract

Rastući trend rezistencije bakterija na antibiotike predstavlja veliki javnozdravstveni problem koji zahtjeva istraživanja novih učinkovitih djelatnih tvari i formulacija. Jedna od takvih bakterija je MRSA, glavni uzročnik bolničkih infekcija s visokim stupnjem mortaliteta. Timol, izoliran iz različitih biljnih vrsta porodice Lamiaceae, pokazao je značajan antimikrobni učinak i dobro djelovanje na MRSU; međutim, zbog svoje lipofilnosti ima ograničenu terapijsku primjenu. S ciljem poboljšanja farmakokinetičkih svojstava i bioraspoloživosti timola, pripremljene su različite fosfolipidne nanoformulacije s uklopljenim timolom kojima su određena fizikalno-kemijska svojstva (veličina liposoma, indeks polidisperznosti, zeta potencijal) i ispitan antimikrobni učinak. Korištenjem visokotlačne homogenizacije pripravljene su fosfolipidne disperzije koje omogućuju visoko uklapanje timola, sa srednjim promjerima vezikula manjim od 200 nm. Ispitivanja in vitro antimikrobne aktivnosti pokazala su da većina pripremljenih fosfolipidnih nanoformulacija s uklopljenim timolom ne pokazuje bolji antimikrobni učinak od otopine timola.

Abstract (english)

The growing trend of antibacterial resistance represents a major public health problem. It requires research of a new antimicrobial substances that should be effective and safe for human use. MRSA is a major cause of hospital-acquired infections with a high mortality rate. Thymol, isolated from different plant species of the Lamiaceae family, showed a significant antimicrobial effect against MRSA. However, due to its lipophilicity, thymol has limited therapeutic use. To improve the pharmacokinetic properties and bioavailability of thymol, it was incorporated into phospholipid-based nanoformulations, vesicular phospholipid gels and conventional liposomes. They were evaluated for the physicochemical properties (mean diameter, polydispersity index, zeta potential) and in vitro antibacterial effect. Vesicular phospholipid gels with thymol, prepared by high-pressure homogenization, resulted with high incorporation of thymol in vesicles smaller than 200 nm. In vitro antibacterial evaluation showed that most of the prepared phospholipid nanoformulations with incorporated thymol did not show a better antimicrobial effect than thymol solution.

• Keywords: fosfolipidi; vezikularni fosfolipidni gel; liposomi; timol; Staphylococcus aureus; MRSA
• Keywords (english): phosholipids; vesicular phospholipid gel; liposomes; thymol; Staphylococcus aureus; MRSA
• Language: croatian
• URN:NBN: urn:nbn:hr:163:476965
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-07-04
• Created on: 2024-09-25 11:53:54