Primjenjivost modificirane QuEChERS metode za detekciju mikotoksina sterigmatocistina u urinu zdravih darivatelja krvi

master's thesis

• Title (english): Applicability of the modified QuEChERS method for the detection of the mycotoxin sterigmatocystin in urine of healthy blood donors
• Author: Matea Pauković
• Mentor: Daniela Jakšić (mentor)
• Committee member: Daniela Jakšić (predsjednik povjerenstva)
• Committee member: Maja Šegvić Klarić (član povjerenstva)
• Committee member: Miranda Sertić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology); Zagreb
• Defense date and country: 2024-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Sterigmatocistin (STC) je kancerogeni i genotoksični mikotoksin kojeg u najvećoj mjeri proizvode ubikvitarne plijesni roda Aspergillus. Mikotoksin je detektiran u brojnim prehrambenim namirnicama, ali i u unutarnjim prostorima sa visokom stopom vlažnosti i poviješću prethodnih poplava. Zabilježeni su brojni mehanizmi kojima ispoljava štetne učinke na organizam, a s obzirom da dijeli biosintetski put i strukturnu sličnost s kancerogenim aflatoksinom B1, potrebna je učinkovita, osjetljiva i dovoljno jednostavna metoda detekcije u biološkim uzorcima. Obradom 94 uzoraka urina zdravih dobrovoljnih darivatelja krvi nastojala se procijeniti izloženost mikotoksinu STC-u. Pritom je korištena optimizirana QuEChERS metoda ekstrakcije i pročišćavanja uzorka. STC je u tragovima detektiran u 18 % uzoraka pri čemu je 61/94 uzoraka obrađen pojednostavljenom QuEChERS metodom, a 38/94 uzoraka je dodatno pročišćeno ekstrakcijom na čvrstoj fazi (SPE). Nije utvrđena statistički značajna razlika u detekciji STC-a s obzirom na metodu pripreme uzoraka. Također nije utvrđen utjecaj dobi i spola ispitanika na prisustvo STC-a u urinu. Dobiveni rezultati pokazali su da u odabranoj zdravoj populaciji dobrovoljnih darivatelja krvi nije utvrđen rizik izloženosti mikotoksinu STC-u. Rezultati također upućuju na prikladnost opisane metode za primjenu u daljnjim istraživanjima usmjerenim na detekciju STC-a u biološkim uzorcima.

Abstract (english)

Sterigmatocystin (STC) is a carcinogenic and genotoxic mycotoxin predominantly produced by ubiquitous molds of the genus Aspergillus. The mycotoxin has been detected in numerous foodstuffs as well as in indoor environments with high humidity and a history of previous flooding. Numerous mechanisms by which it exerts harmful effects on the organism have been documented. Given that it shares a biosynthetic pathway and structural similarity with the carcinogenic aflatoxin B1, there is a need for an effective, sensitive, and sufficiently simple method for its detection in biological samples. By analyzing 94 urine samples from healthy blood donor volunteers, we aimed to assess exposure to the mycotoxin STC. An optimized QuEChERS method for extraction and sample purification was employed. STC was detected in trace amounts in 18 % of samples, with 61/94 samples processed using the simplified QuEChERS method, and 38/94 samples further purified using solid-phase extraction (SPE). No statistically significant difference was found in the detection of STC with respect to the sample preparation method. Additionally, the influence of age and gender on the presence of STC in urine was not statistically significant. The obtained results indicated that there was no risk of STC exposure in the selected healthy population of blood donor volunteers. The results also indicate the suitability of the described method for application in further research aimed at detecting STC in biological samples.

• Keywords: biološki uzorci; procjena izloženosti; sterigmatocistin; urin; QuEChERS
• Keywords (english): assesssment of exposure; biological samples; sterigmatocystin; urine; QuEChERS
• Language: croatian
• URN:NBN: urn:nbn:hr:163:611414
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-26 09:59:35