Određivanje teških metala u dodacima prehrani za liječenje upalnih bolesti crijeva GFAAS tehnikom

master's thesis

• Title (english): Determination of heavy metals in dietary supplement therapies for inflammatory bowel disease by graphite furnace atomic absorption spectrometry
• Author: Lucija Smrkulj
• Mentor: Daniela Amidžić Klarić (mentor)
• Committee member: Daniela Amidžić Klarić (predsjednik povjerenstva)
• Committee member: Sandra Šupraha-Goreta (član povjerenstva)
• Committee member: Petra Turčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2024-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Uključivanje dodataka prehrani standardnoj terapiji za liječenje upalnih bolesti crijeva može pozitivno djelovati na tijek i jačinu bolesti. Kurkumin, andrografolidi, piperin i bosvelične kiseline su biološki aktivne tvari biljnog podrijetla koje svojim protuupalnim, antioksidativnim, i/ili imunomodulatornim svojstvima smanjuju simptome bolesti. Cilj ovog rada bio je procijeniti kvalitetu i sigurnost dodataka prehrani s obzirom na sadržaj teških metala; arsena, kadmija i olova. Budući da su navedeni metali najčešće zastupljeni u niskim koncentracijama, odabrana je tehnika grafitne atomske apsorpcijske spektrometrije nakon postupka mikrovalne digestije. Dobiveni rezultati upućuju na niski sadržaj metala arsena i kadmija. Međutim, sadržaj olova u dva uzorka prelazi dozvoljenu dnevnu izloženost oralnom putem te njihova primjena predstavlja rizik za zdravlje ljudi. U zaključku se ističe potreba stalnog praćenja sigurnosti dodataka prehrani dostupnih na tržištu s obzirom na sadržaj olova.

Abstract (english)

The inclusion of dietary supplements in the standard therapy for the treatment of inflammatory bowel diseases can have a positive effect on the course and severity of the disease. Curcumin, andrographolides, piperine and boswellic acids are biologically active compounds from plants that reduce disease symptoms with their anti-inflammatory, antioxidant and/or immunomodulatory properties. The aim of this work was to evaluate the quality and safety of dietary supplements with regard to the content of heavy metals such as arsenic, cadmium and lead. Since the mentioned metals are primarily present in low concentrations, the graphite atomic absorption spectrometry technique was used after the microwave digestion procedure. The results indicated a low content of metals, including arsenic and cadmium. However, the lead content in two samples exceeds the permitted daily oral exposure, and their use could pose a risk to human health. In conclusion, the need for constant monitoring of the safety of dietary supplements available on the market regarding lead content is emphasized.

• Keywords: arsen; olovo; kadmij; dodaci prehrani; upalne bolesti crijeva; kurkuma; justicija; indijski tamjanovac; crni papar; GFAAS
• Keywords (english): arsenic; cadmium; lead; dietary supplements; inflammatory bowel diseases; Turmeric; Green chiretta; Indian frankincense; Black pepper; GFAAS
• Language: croatian
• URN:NBN: urn:nbn:hr:163:512227
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2026-07-15
• Created on: 2024-09-27 11:35:44