Ispitivanje biokompatibilnosti deformabilnih liposoma s azitromicinom uklopljenih u kitozanski i karbopolski gel

master's thesis

• Title (english): Biocompatibility study of azithromycin-loaded deformable liposomes incorporated into chitosan and carbopol gel
• Author: Sara Hulak
• Mentor: Zora Rukavina (mentor)
• Committee member: Zora Rukavina (predsjednik povjerenstva)
• Committee member: Laura Nižić Nodilo (član povjerenstva)
• Committee member: Emerik Galić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2024-09-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Prilikom razvoja hibridnih formulacija liposomi-u-gelu, potrebno je voditi računa ne samo o
učinkovitosti i biofarmaceutskim karakteristikama formulacije, već i o biokompatibilnosti s
odabranim staničnim kulturama. U sklopu ovog istraživanja deformabilni liposomi s AZT-om (DL)
uklopljeni su u 2 različita gela: kitozanski gel (CHG) i karbopolski gel (CAG), s ciljem ispitivanja i
usporedbe biokompatibilnosti dobivenih formulacija liposomi-u-gelu in vitro na HaCaT staničnoj
liniji. Formulacija DL-CHG je u rasponu koncentracija ≤ 32 μg/ml biokompatibilna s HaCaT
stanicama, dok je pri koncentraciji  64 μg/ml vijabilnost stanica bila ˂ 70 % te se može smatrati
citotoksičnom za HaCaT stanice. U usporedbi s formulacijom DL-CHG, DL-CAG pokazuje
biokompatibilnost u širem rasponu koncentracija (0,25  64 μg/ml), te je pri najvišoj testiranoj
koncentraciji vijabilnost tretiranih stanica veća u odnosu na formulaciju DL-CHG pri istoj
koncentraciji, zbog čega se CAG može smatrati prikladnijom podlogom za uklapanje DL od CHG.
Budući da su se gelovi s uklopljenim neliposomskim lijekom također pokazali citotoksičnima pri
visokim koncentracijama AZT-a, čini se da citotoksičnost formulacija DL-u-gelu pri koncentraciji
 64 μg/ml proizlazi djelomično iz prisutnosti samog AZT-a u formulaciji, a djelomično je odgovoran
sastav liposomske formulacije DL.

Abstract (english)

During the development of hybrid liposome-in-gel formulations, it is essential to consider not
only the efficacy and biopharmaceutical characteristics of the formulation but also biocompatibility
with selected cell cultures. In this study, AZT-loaded deformable liposomes (DL) were incorporated
into two different gels: chitosan gel (CHG) and carbopol gel (CAG), with the aim of testing and
comparing the biocompatibility of the obtained liposome-in-gel formulations in vitro on the HaCaT
cell line. The DL-CHG formulation was biocompatible with HaCaT cells at a concentration ≤ 32
μg/ml, while at a concentration ≥ 64 μg/ml, cell viability was ˂ 70%, which can be considered
cytotoxic. Compared with the DL-CHG formulation, DL-CAG shows biocompatibility over a wider
range of concentrations (0.25  64 μg/ml). Moreover, at the highest tested concentration, the viability
of treated cells was greater compared to the DL-CHG formulation, making CAG a more suitable
vehicle for incorporating DL from a biocompatibility standpoint. Since gels with incorporated nonliposomal
AZT also exhibited cytotoxicity at high concentrations, it seems that the cytotoxicity of the
DL-in-gel formulations at concentrations ≥ 64 μg/ml arises partly from the presence of AZT itself in
the formulation, and partly from the composition of the DL liposome formulation.

• Keywords: deformabilni liposomi; azitromicin; karbopolski gel; kitozanski gel; biokompatibilnost; HaCaT stanice
• Keywords (english): deformable liposomes; azithromycin; carbopol gel; chitosan gel; biocompatibility; HaCaT cells
• Language: croatian
• URN:NBN: urn:nbn:hr:163:745741
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-14 12:23:15