Ispitivanje membranske elastičnosti vezikularnih fosfolipidnih gelova s ciprofloksacinom i timolom

master's thesis

• Title (english): Evaluation of the membrane elasticity of various vesicular phospholipid gels containing ciprofloxacin or thymol
• Author: Vita Vuković
• Mentor: Željka Vanić (mentor)
• Committee member: Željka Vanić (predsjednik povjerenstva)
• Committee member: Maja Bival Štefan (član povjerenstva)
• Committee member: Laura Nižić Nodilo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2024-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Svrha ovog rada bila je odrediti membransku elastičnost vezikularnih fosfolipidnih gelova (VFG-ova) s ciprofloksacinom i timolom različitog (fosfo)lipidnog sastava. Formulacije VFG-ova pripravljene su metodom visokotlačne homogenizacije te su se međusobno razlikovale (fosfo)lipidnim sastavom, prisustvom suotapala (propilenglikol), rubnog aktivatora (monoacil fosfatidilkolin) i polimera (kitozan). Ispitivan je utjecaj sastavnica VFG-a na fluidnost (elastičnost/rigidnost) njegovih dvoslojeva. Najelastičnije dvoslojeve imali su VFG-ovi s ciprofloksacinom koji su uz osnovni fosfolipid (SPC) sadržavali rubni aktivator (monoacil fosfatidilkolin) i propilenglikol, potom VFG-ovi s propilenglikolom te oni koji su uz SPC sadržavali monoacil fosfatidilkolin. Nasuprot tome, prisutnost kolesterola, kitozana i hidrogeniranih fosfolipida rezultirala je porastom rigidnosti membrana VFG-ova, pri čemu su VFG-ovi s hidrogeniranim fosfolipidima bili karakterizirani najčvršćim dvoslojevima (najmanji stupanj elastičnosti). Osim sastavnica VFG-a, dokazano je i da lipofilnost uklopljenog lijeka utječe na stupanj membranske elastičnosti. Pritom su membrane VFG-ova s timolom bile nešto elastičnije od dvoslojeva VFG-ova s ciprofloksacinom istog (fosfo)lipidnog sastava.

Abstract (english)

The purpose of this study was to determine membrane elasticity of vesicular phospholipid gels (VPGs) containing ciprofloxacin or thymol. VPGs, differing in (phospho)lipid composition, with or without addition of a co-solvent (propylene glycol), an edge activator (monoacyl phosphatidylcholine) and a polymer (chitosan), were prepared using high-pressure homogenization method. The influence of the VPGs' ingredients on the fluidity (elasticity/rigidity) of their bilayers was examined. Ciprofloxacin-VFGs, which in addition to the basic phospholipid (SPC) contained an edge activator (monoacyl phosphatidylcholine) and propylene glycol, were characterized by the most elastic bilayers, followed by VPGs containing propylene glycol and those containing SPC and monoacyl phosphatidylcholine. On the other hand, the presence of cholesterol, chitosan, and hydrogenated phospholipids resulted with increase in the membrane rigidity, where VPG containing hydrogenated phospholipids exhibited the most rigid bilayers. In addition to VPG ingredients, the lipophilicity of the incorporated drug was also shown to influence the degree of membrane elasticity. In this regard, VPGs with thymol were slightly more elastic than those with ciprofloxacin of the same (phospho)lipid composition.

• Keywords: vezikularni fosfolipidni gel; membranska elastičnost; liposomi; ciprofloksacin; timol
• Keywords (english): vesicular phospholipid gel; membrane elasticity; liposomes; ciprofloxacin; thymol
• Language: croatian
• URN:NBN: urn:nbn:hr:163:466780
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-07-23
• Created on: 2025-01-08 14:35:52