Utjecaj bisfenola A na antioksidacijska i antidijabetska svojstva običnog luka (Allium cepa L.)

master's thesis

• Title (english): The impact of bisphenol A on the antioxidant and antidiabetic properties of common onion (Allium cepa L.)
• Author: Tena Špoljarić
• Mentor: Ana-Marija Domijan (mentor)
• Committee member: Ana-Marija Domijan (predsjednik povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Committee member: Lovorka Vujić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical botany); Zagreb
• Defense date and country: 2025-02-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Bisfenol A (BPA) koristi se kao sirovina u proizvodnji epoksidnih smola i plastike. Proizvodnja i odlaganja takvih proizvoda u okolišu dovelo je do sve veće prisutnost BPA u okolišu. Pokazano je da BPA negativno utječe na zdravlje ljudi tako što djeluje kao endokrini disruptor te uzrokuje oksidacijski stres. Cilj ovog istraživanja bio je ispitati učinak BPA na antioksidacijsku i antidijabetsku aktivnost u lukovica običnog luka (Allium cepa L.). Pokus je proveden tako da su 5 skupina po 10 lukovica luka bile izložene otopinama BPA u rasponu koncentracija od 1 do 50 mg/L tijekom tri dana. Jedna je skupina lukovica (n=10) bila izložena destiliranoj vodi te je služila kao kontrola. Antioksidacijska aktivnost lukovica luka ispitana je FRAP, DPPH i ABTS metodama, a antidijabetska aktivnost ispitana je metodom BSA glikacije. Rezultati istraživanja pokazali su da trodnevna izloženost BPA dovodi do pad redukcije željezovih iona i inhibicije slobodnih radikala u usporedbi s kontrolom što ukazuje na to da BPA smanjuje antioksidacijsku aktivnost biljke. Također, u usporedbi s kontrolom trodnevna izloženost najvišoj koncentraciji BPA smanjila je sposobnost inhibicije glikacije proteina biljke. Ovo istraživanje potvrđuje štetno djelovanje BPA na biljke tako što dovodi do smanjenja njihove antioksidacijske i antidijabetske aktivnosti te ukazuje na važnost kontrole količine BPA u okolišu kako bi se smanjili njegovi štetni učinci na okoliš i zdravlje ljudi.

Abstract (english)

Bisphenol A (BPA) is used as a raw material in the production of epoxy resins and plastics. Production and release of such products in the environment has led to increased presence of BPA in the environment. It is demonstrated that BPA has negative effects on human health by acting as an endocrine disruptor and causing oxidative stress. The aim of this study was to investigate the impact of BPA on antioxidant and antidiabetic activities of onion bulbs (Allium cepa L.). In the experiment five groups (each consisting of 10 bulbs) were exposed to BPA at concentrations from 1 to 50 mg/L for three days. One group of onions (n=10 bulbs) was exposed to distilled water and served as control. Following exposure, in onion bulbs antioxidant activity was evaluated using the FRAP, DPPH, and ABTS methods, while antidiabetic activity was assessed using the BSA glycation method. The results of this investigation showed that three-day exposure to BPA caused a decrease in iron ion reduction and free radical inhibition in comparison to the control, indicating that BPA decreases the antioxidant activity of the plants. Also, in comparison to the control three-day exposure to the highest BPA concentration reduced the plants’ ability to inhibit protein glycation. This study confirms the harmful effects of BPA on plants by reducing their antioxidative and antidiabetic activity and underscores the importance of regulating the amount of BPA released into the environment to minimize its adverse effects on the environment and human health.

• Keywords: bisfenol A; Allium cepa; antioksidacijska aktivnost; antidijabetska aktivnost; FRAP; DPPH; ABTS; BSA glikacija; polifenoli
• Keywords (english): bisphenol A; Allium cepa; antioxidant activity; antidiabetic activity; FRAP; DPPH; ABTS; BSA glycation; polyphenols
• Language: croatian
• URN:NBN: urn:nbn:hr:163:918866
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-04-02 10:47:06