Određivanje željeza i mangana u dodacima prehrani za liječenje upalnih bolesti crijeva atomskom apsorpcijskom spektrometrijom

master's thesis

• Title (english): Determination of iron and manganese in dietary supplements for the treatment of inflammatory bowel disease by atomic absorption spectrometry
• Author: Mirjana Lasić
• Mentor: Daniela Amidžić Klarić (mentor)
• Committee member: Daniela Amidžić Klarić (predsjednik povjerenstva)
• Committee member: Ana Mornar Turk (član povjerenstva)
• Committee member: Snježana Zubčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2025-04-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

S obzirom na sve učestaliju pojavnost upalnih bolesti crijeva, raste i interes za dodatke prehrani
koji se koriste kao potporna terapija u njihovom liječenju. Budući da su dodaci prehrani u
Europskoj uniji regulirani kao hrana, a ne kao lijekovi, njihova je kontrola manje stroga, što ističe
važnost neovisnog ispitivanja njihove kvalitete. U ovom su radu analizirani dodaci prehrani koji
sadrže kurkumu, indijski tamjanovac, crni papar i justiciju. Ukupno je analizirano petnaest uzoraka
kupljenih u trgovinama zdrave hrane i putem internetske prodaje. Nakon mikrovalne digestije,
sadržaj željeza i mangana određen je metodom atomske apsorpcijske spektrometrije. Rezultati su
pokazali varijabilnost u koncentracijama ispitivanih metala među različitim pripravcima, pri čemu
su najviše vrijednosti zabilježene u uzorcima justicije i indijskog tamjanovca. Dobiveni rezultati
u skladu su s podacima iz dostupne literature, što potvrđuje pouzdanost korištene metode analize.
Analizirani dodaci prehrani pokazali su se kao vrijedan izvor željeza i mangana te potencijalno
mogu pridonijeti smanjenju nutritivnih deficita kod osoba s upalnim bolestima crijeva. Njihova
uloga u nadopunjavanju prehrane i očuvanju zdravlja dodatno potvrđuje potrebu za redovitom
kontrolom njihove kakvoće i sigurnosti.

Abstract (english)

Given the increasing incidence of inflammatory bowel diseases (IBD), there is growing interest
in dietary supplements used as supportive therapy in their treatment. Since dietary supplements
are regulated as food in the European Union, rather than as medicines, their quality control is less
stringent, highlighting the importance of independent product testing. This study analyzed dietary
supplements containing turmeric, Indian frankincense, black pepper, and Green chiretta. A total
of fifteen samples were examined, purchased from health food stores and via online retailers.
Following microwave-assisted digestion, the content of iron and manganese was determined using
flame atomic absorption spectrometry. The results showed variability in the concentrations of the
analyzed trace metals among the different supplements, with the highest levels found in samples
containing Green chiretta and Indian frankincense. The obtained values were consistent with data
reported in the scientific literature, confirming the reliability of the analytical method used. The
analyzed dietary supplements proved to be valuable sources of iron and manganese and may help
reduce deficiencies of these essential micronutrients in individuals with IBD. Their role in
complementing the daily diet and maintaining health underlines the need for regular quality and
safety control of such products.

• Keywords: željezo; mangan; dodaci prehrani; upalne bolesti crijeva; kurkuma; justicija; indijski tamjanovac; crni papar; atomska apsorpcijska spektrometrija
• Keywords (english): iron; manganese; dietary supplements; inflammatory bowel diseases; turmeric; green chiretta; Indian frankincense; black pepper; atomic absorption spectrometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:209055
• Study programme: Title: Pharmacy - integrated undergraduate and graduate - university study; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni magistar farmacije (sveučilišni magistar farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2027-04-14
• Created on: 2025-04-24 09:37:40