Kvalitativna i kvantitativna analiza polifenola vrsta Stachys iva Griseb. i Stachys horvaticii Micevski, Lamiaceae

master's thesis

• Title (english): Qualitative and quantitative analysis of polyphenols of Stachys iva Griseb. and Stachys horvaticii Micevski, Lamiaceae
• Author: Ivana Vukičević
• Mentor: Renata Jurišić Grubešić (mentor)
• Committee member: Renata Jurišić Grubešić (predsjednik povjerenstva)
• Committee member: Jadranka Vuković Rodriguez (član povjerenstva)
• Committee member: Željka Vanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2016-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U ovom je diplomskom radu provedena kvalitativna i kvantitativna analiza polifenolnih tvari u
nadzemnim dijelovima vrsta Stachys iva Griseb. i S. horvaticii Micevski (Lamiaceae). Prisutnost
polifenolnih tvari dokazana je općim reakcijama stvaranja obojenih produkata ili taloga.
Kvantitativna analiza ukupnih polifenola (UP), trjeslovina (T), flavonoida (F) i fenolnih kiselina
(FK) provedena je spektrofotometrijskim metodama. Sadržaj analiziranih polifenolnih tvari za
vrstu Stachys iva iznosio je: 6,03±0,03% (UP); 1,70±0,01% (T); 0,15±0,00% (F) i 1,65±0,02%
(FK), a za vrstu S. horvaticii: 3,80±0,02% (UP); 0,78±0,03% (T); 0,43±0,00% (F) i 0,74±0,01%
(FK). Vrsta Stachys iva imala je dvostruko više ukupnih polifenola, trjeslovina i fenolnih kiselina
od vrste S. horvaticii, dok je u vrste S. horvaticii izmjereno više flavonoida. Provedena
fitokemijska karakterizacija predstavlja prilog znanstvenom istraživanju roda Stachys te
upotpunjuje dosadašnje spoznaje o fitoterapijskom potencijalu vrsta Stachys iva Griseb. i S.
horvaticii Micevski (Lamiaceae).

Abstract (english)

In this work, qualitative and quantitative analysis of polyphenols in aerial parts of Stachys iva Griseb.
and S. horvaticii Micevski was carried out. The presence of polyphenols was proven by using general
reactions of developing colored products and precipitates. Quantitative analyses of total polyphenols
(TP), tannins (T), flavonoids (F), and phenolic acids (PA) were carried out using spectrophotometric
methods. The contents of analyzed polyphenolic compounds in Stachys iva were as follows:
6.03±0.03% (UP); 1.70±0.01% (T); 0.15±0.00% (F), and 1.65±0.02% (FK), and in S. horvaticii:
3.80±0.02% (UP); 0.78±0.03% (T); 0.43±0.00% (F), and 0.74±0.01% (FK). Stachys iva had twice the
total polyphenols, tannins, and phenolic acids than S. horvaticii, while S. horvaticii had more
flavonoids. Performed phytochemical characterization is a contribution to the scientific study of the
genus Stachys, and also contributes to the existing knowledge concerning phytotherapeutic potential of
Stachys iva Griseb. and S. horvaticii Micevski (Lamiaceae).

• Keywords: Stachys iva Griseb.; S. horvaticii Micevski; polifenoli; trjeslovine; flavonoidi; fenolne kiseline; kvalitativna analiza; kvantitativna analiza; UV-Vis spektrofotometrija
• Keywords (english): Stachys iva Griseb.; S. horvaticii Micevski; total polyphenols; tannins; flavonoids; phenolic acids; qualitative analysis; quantitative analysis; UV-Vis spectrophotometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:592190
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-11-23 15:32:11