Uloga astrocita u eksperimentalnom modelu kronične mišićne boli uzrokovane karagenanom

master's thesis

• Title (english): The role of astrocytes in experimental model of chronic carrageenan-induced muscle pain
• Author: Ivona Barišić
• Mentor: Lidija Bach-Rojecky (mentor)
• Committee member: Lidija Bach-Rojecky (predsjednik povjerenstva)
• Committee member: Iva Mucalo (član povjerenstva)
• Committee member: Ivan Pepić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacology); Zagreb
• Defense date and country: 2016-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Upalna bol javlja se nakon upale ili ozljede tkiva prilikom čega dolazi do lučenja upalnih medijatora koji aktiviraju nociceptore. Periferne promjene uzrokovane upalom dovode do senzitizacije neurona u kralješničnoj moždini. Ti procesi, poznati kao periferna i središnja senzitizacija, doprinose prelasku akutne boli u kroničnu.
Električni impulsi nastali podraživanjem nociceptora na periferiji dolaze u dorzalni rog kralješnične moždine, gdje se nalaze astrociti. To su glija stanice zvjezdastog oblika za koje je dokazano da se aktiviraju u modelima kronične boli i otpuštaju medijatore koji doprinose procesu središnje senzitizacije.
U ovom diplomskom radu mjeren je stupanj aktivacije astrocita imunohistokemijskom metodom u uzorcima kralješnične moždine pokusnih životinja kojima je uzrokovana kronična bol mišićnog porijekla injiciranjem 3%-tne otopine karagenana. Imunohistokemijskom analizom pokazali smo da nema statistički značajnog povećanja ekspresije markera aktivacije astrocita (GFAP) u dorzalnom rogu kralješnične moždine 21 dan nakon početne ozljede mišića. Dobiveni rezultati dovode do zaključka da, za razliku od kronične neuropatske boli, astrociti nemaju bitnu ulogu u nastajanju i održavanju kronične boli mišićnog porijekla.
Potrebna su daljnja istraživanja uključenosti astrocita u procese razvoja kronične boli koja bi u obzir uzela promatranja njihove aktivacije u različitim vremenima od početne ozljede dubokih tkiva te interakciju s mikroglijom i neuronima. Rezultati tih istraživanja bi u konačnici mogli usmjeriti razvoj potencijalnih novih analgetika za liječenje kroničnih boli na modulaciju funkcije glija stanica u središnjem živčanom sustavu.

Abstract (english)

Inflammatory pain occurs as a result of inflammation or tissue injury which causes the secretion of various inflammatory mediators that activate nociceptors. Peripheral changes caused by inflamation result in sensitization of spinal cord neurons. Those processes, known as peripheral and central sensitization, contribute to the transition from acute to chronic pain.
Electrical impulses generated by activated nociceptors on periphery are transmitted to the dorsal horn of the spinal cord,where are placed astrocytes. They are a star-shaped glia cells activated in experimental models of chronic pain and they release mediators that make contribution to the process of central sensitization.
This thesis is based on measurement of astrocyte activation, using an immunohistochemical method, in tissue samples of dorsal horn of the spinal cord of animals which had previously caused chronic muscle pain by using 3% carrageenan solution. Using an immunohistochemical analysis we revealed that there is no statistically significant increase in expression of astrocytes marker (GFAP) in the dorsal horn of the spinal cord 21 day after the initial muscle injury. These results lead us to the conclusion that, unlike the chronic neuropathic pain, astrocytes do not have an important role in appearance and maintenance of chronic muscle pain.
Further researches about the involvement of astrocytes in processes of development of chronic pain are needed. They should consider activation of astrocytes in different times after initial injury of deep tissues and their interaction with microglia and neurons. The results of those researches could direct development od new analgetic drugs for chronic pain on modulation of function of glia cells in central nervous system.

• Keywords: bol; upalna bol; astrociti; imunohistokemija; GFAP
• Keywords (english): pain; inflammatory pain; astrocytes; immunohistochemistry; GFAP
• Language: croatian
• URN:NBN: urn:nbn:hr:163:296762
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-12-02 14:42:51