Utjecaj ciklodekstrina na antioksidacijsku učinkovitost ekstrakta komine masline

master's thesis

• Title (english): The impact of cyclodextrins on the antioxidant efficiency of the olive pomace extract
• Author: Markonija Spaić
• Mentor: Dubravka Vitali Čepo (mentor)
• Committee member: Dubravka Vitali Čepo (predsjednik povjerenstva)
• Committee member: Mario Jug (član povjerenstva)
• Committee member: Petra Turčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of food chemistry); Zagreb
• Defense date and country: 2016-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Nakon proizvodnje maslinovog ulja zaostaje velika količina komine čije direktno odlaganje u okoliš predstavlja veliki ekološki problem. Ponovno iskorištavanje komine masline za izolaciju bioaktivnih komponenata i naknadno za proizvodnju energije izvrsna je mogućnost za održivo zbrinjavanje otpada kojom se ostvaruje i ekološka i ekonomska korist. Naime, polifenoli komine posjeduju dokazano protuupalno i antikancerogeno djelovanje te je stoga komina izvrsna sirovina za izolaciju bioaktivnih sastavnica koje se kasnije koriste kao prirodni aditivi i nutraceutici u farmaceutskoj i prehrambenoj industriji. Upravo je zbog toga važno razviti učinkovite i ekološki prihvatljive postupke izolacije i formulacije ekstrakata komine što je zahtjevan proces zbog heterogenosti i kompleksnosti sastava same sirovine kao i vrlo loših tehnoloških parametara dobivenih ekstrakta. U ovom radu istražen je utjecaj uklapanja polifenola komine u inkluzijske komplekse s različitim ciklodekstrinima na različite tipove antioksidacijske aktivnosti. U tom smislu istražen je utjecaj različitih koncentracijskih nivoa β-ciklodekstrina, HP-β-ciklodekstrina, metil-β-ciklodekstrina i γ-ciklodekstrina na antiradikalnu učinkovitost protiv ABTS i DPPH radikala te utjecaj na redukcijski kapacitet dobivenih ekstrakta. Obzirom na dobivene rezultate β-ciklodekstrin i metil- β-ciklodekstrin mogu se smatrati ciklodekstrinima izbora u formulaciji suhog ekstrakta komine masline.

Abstract (english)

There is a large amount of pomace remaining after olive oil production , whose direct disposal into the environment is a major environmental problem. Re-use of olive pomace for the isolation of bioactive compounds and subsequent energy production is an excellent possibility for sustainable waste management which generates both, environmental and economic benefits. The pomace polyphenols have shown anti-inflammatory and anti-cancer effects and therefore pomace is excellent raw material for the isolation of bioactive components that are later used as natural additives and nutraceuticals in the pharmaceutical and food industries. Developing efficient and environmentally friendly methods of isolation and formulation of pomace extracts is a challenging process due to the heterogeneity and complexity of the composition of raw material and very poor technological parameters of obtained raw extract. This thesis examined the influence of incorporating pomace polyphenols in inclusion complexes with different cyclodextrins on different types of antioxidant activity of obtained products. More specificaly, the influence of different concentration levels of β-cyclodextrin, HP-β-cyclodextrin, methyl-β-cyclodextrin and γ-cyclodextrin on the antiradical activity against DPPH and ABTS radicals and reducing activity of obtained extracts was investigated. d. Based on the obtained results, β-cyclodextrin and methyl-β-cyclodextrin can be considered as excipients of choice in the formulationpomace polyphenol extract.

• Keywords: komina masline; fenoli; ciklodekstrini; antioksidacijska aktivnost
• Keywords (english): olive pomace; phenols; cyclodextrins; antioxidant activity
• Language: croatian
• URN:NBN: urn:nbn:hr:163:483396
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-12-02 16:41:31