Određivanje aktivnosti FXI u 2. internacionalnom standardu i aktivnosti FXII u 1. internacionalnom standardu Svjetske zdravstvene organizacije

master's thesis

• Title (english): Assignment of functional activity of FXI for 2nd international and activity of FXII for 1st international World health organization standard
• Author: Anamarija Rade
• Mentor: Renata Zadro (mentor)
• Committee member: Renata Zadro (predsjednik povjerenstva)
• Committee member: Roberta Petlevski (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2016-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Sustav zgrušavanja omogućuje brz i efikasan odgovor na vaskularnu ozljedu stvaranjem fibrinskog ugruška. Proces se sastoji od niza kaskadnih reakcija i interakcija u kojima sudjeluju plazmatski glikoproteini koje sintetizira jetra, a poznatiji su kao faktori zgrušavanja. Manjak ili neodgovarajuća aktivnost pojedinih faktora može dovesti do poremećaja hemostaze pa je u dijagnostičke svrhe bitno određivanje aktivnosti pojedinih faktora u plazmi. Aktivnost faktora određuje se koagulometrijskom metodom mjerenjem modificiranog APTV-a ili PV-a u reakciji gdje su svi faktori u suvišku osim mjerenog faktora tako da brzina zgrušavanja ovisi o aktivnosti mjerenog faktora. Kako se radi o komparativnim metodama potrebno je razviti standarde poznate aktivnosti faktora koji će se koristiti za umjeravanje uređaja. WHO IS standardi su primarni standardi i ne koriste se u rutinskoj upotrebi već im je osnovna svrha umjeravanje sekundarnih radnih standarda.
Ovaj rad opisuje sudjelovanje u kolaborativnoj studiji NIBSC koja je obuhvatila 29 laboratorija u 11 država diljem svijeta, a predlaže dva uzorka za 2. WHO IS standard za aktivnost FXI i 1. WHO IS standard za aktivnost FXII. Oba dobivena uzorka su analizirana i određena je aktivnost FXI i FXII te su rezultati poslani u NIBSC na statističku obradu. Iz konačnog izvješća saznajemo da se radilo o dva ista uzorka te da je predložena aktivnost FXI u novom standardu 0,71 kIU/L, a za FXII nismo dobili izvješće jer se radilo o studiji izvedivosti.

Abstract (english)

Coagulation system enables quick and efficient response to a vascular injury by the production of fibrin clot. The process consists of a series of cascade reactions and interactions that involve plasma glycoproteins, which are synthesized by the liver and known as coagulation factors. The deficiency or inadequate activity of certain factors can lead to hemostasis disorder, which is why it is important to determine the activity of particular factors in plasma. The factor activity is determined by coagulometric assay that measures the modified APTT or PT in a reaction in which all factors are in excess, except for the factor whose activity we are measuring, so the speed of clot formation depends on the activity of the measured factor only. As we are using comparative methods, it is necessary to develop standards of the given activity that will be used to calibrate the instrument. WHO IS standards are not used for routine testing, but their primary function is the calibration of secondary working standards.
This study describes the participation in the NIBSC collaborative study, which included 29 laboratories in 11 countries around the world, and proposes two samples for the 2nd international standard for blood coagulation factor XI and the 1st international standard for blood coagulation factor XII. Having analysed both samples and having determined the activity of FXI and FXII, we sent the results to NIBSC for statistical analysis. From the final report, we learned that the two samples were duplicates and that the proposed activity for FXI is 0.71 kIU/L. As for FXII, the report was not given because it was measured within a feasibility study.

• Keywords: FXI; FXII; internacionalni standardi; Svjetska zdravstvena organizacija
• Keywords (english): FXI; FXII; international standards; World Health Organization
• Language: croatian
• URN:NBN: urn:nbn:hr:163:546431
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-12-06 14:41:50