ABO blood group genotypes in women with breast cancer

master's thesis

• Title: Genotipovi ABO sustava krvnih grupa kod žena oboljelih od tumora dojke
• Author: Tamara Bezek
• Mentor: Roberta Petlevski (mentor)
• Committee member: Roberta Petlevski (predsjednik povjerenstva)
• Committee member: Sandra Šupraha Goreta (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2016-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

ABO sustav krvnih grupa povezan je s rizikom od nekoliko malignih bolesti, ali još uvijek nije razjašnjeno je li rizik od tumora dojke veći kod nositeljica određene ABO krvne grupe. Cilj istraživanja bio je ispitati povezanost između genotipova ABO krvnih grupa i pojave tumora dojke te ispitati povezanost između genotipova ABO krvnih grupa i zastupljenosti gradusa tumora (I-III) i biljega ER, PR i HER2/neu. Istraživanjem je obuhvaćeno 59 bolesnica kojima je dijagnosticiran invazivni tumor dojke i 80 asimptomatskih, zdravih žena, dobrovoljnih darivateljica krvi. Genomska DNA izolirana je iz pune krvi pomoću komercijalno dostupnog paketa reagensa za izolaciju: QIAampDNA Blood Mini Kit (QIAGEN, Njemačka). Genotipizacija na pet osnovnih alela (O1, O2, A1, A2, B) i 15 genotipova bila je izvedena pomoću PCR-SSP metode. Usporedbom genotipova i fenotipova ABO krvne grupe između pacijentica i kontrolne skupine, dobiveno je P>0,05 te nema statističke značajnosti za povezanost ABO genotipova/fenotipova između skupine pacijentica koje boluju od tumora dojke i kontrolne skupine. Ispitivanjem značajnosti u razlici zastupljenosti gradusa tumora, statusa ER, PR i HER2/neu nisu dobivene statistički značajne vrijednosti za pojavu određenog gradusa tumora te statusa biljega ER, PR i HER2/neu u genotipu O1A1 u odnosu na ne-O1A1 genotipove ABO sustava krvnih grupa. Rezultati rada potvrdili su da ne postoji povezanost između genotipova/fenotipova ABO krvnih grupa i tumora dojke kod ispitivanih skupina.

Abstract (english)

ABO blood type is associated with risk for several malignancies, but still is not clear if the risk of breast cancer is greater in carriers of certain ABO blood group. The aim of this study was to examine the correlation between ABO blood group genotypes and the occurrence of breast cancer, as well as the association between ABO blood group genotypes and the presence of certain tumor grades (I-III) and ER, PR, HER2 markers. The research included 59 patients who were diagnosed with invasive breast cancer and 80 asymptomatic, healthy women,voluntary blood donors. Genomic DNA was isolated from whole blood using commercially available reagent packages for isolation QIAampDNA Blood Mini Kit (QIAGEN, Germany). Genotyping of the five basic alleles (O1, O2, A1, A2, B) and 15 genotypes was performed using PCR-SSP method. By comparing genotypes and phenotypes of ABO blood groups between the patients and the control group, P>0,05 was obtained and there is no statistical significance for the correlation between ABO genotypes/phenotypes of the patient group and the control group. By examining the significance of different tumor grade occurrence, and ER, PR and HER2/neu status, it was discovered that there is no statistical significance for the appearance of a particular grade, and tumor markers ER status, PR and HER2 / neu status in O1A1 genotype compared to non-O1A1 genotypes of ABO blood group system. The thesis results confirmed that there is no correlation between ABO blood type genotypes/phenotypes and breast cancer in tested groups.

• Keywords: ABO krvne grupe; tumor dojke; ABO genotipovi; ABO fenotipovi
• Keywords (english): ABO blood groups; breast cancer; ABO genotypes; ABO phenotypes
• Language: croatian
• URN:NBN: urn:nbn:hr:163:395009
• Study programme: Title: Medicinska biokemija; Study programme type: sveučilišni; Study level: integrirani preddiplomski i diplomski; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Restricted access
• Created on: 2016-12-06 14:54:04