Izolacija antioksidativnih sastavnica biljke Medicago sativa L. primjenom ciklodekstrina

master's thesis

• Title (english): Isolation of antioxidant components of Medicago sativa L. using cyclodextrins
• Author: Sara Šimić
• Mentor: Mario Jug (mentor)
• Committee member: Mario Jug
• Committee member: Jasna Jablan
• Committee member: Željka Vanić
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2015-07-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Biljka lucerna (Medicago sativa L.) nalazi široku primjenu u ljekovite svrhe. Posjeduje antimikrobno, protuupalno, antikancerogeno i antioksidativno djelovanje zbog prisutnosti fenolnih komponenti koje se ponašaju kao slobodni radikali i kelatori metala. Za ekstrakciju fenolnih spojeva iz biljaka najčešće su korištena organska otapala koja negativno utječu na okoliš i ljude. Stoga je cilj ovog diplomskog rada bio optimizirati uvjete ekstrakcije fenolnih komponenti iz vrste Medicago sativa L. korištenjem vodene otopine hidroksipropil-β-ciklodekstrina. Korištenjem programa za statističko planiranje eksperimenta uspoređen je utjecaj različitih procesnih parametara na učinkovitost ekstrakcije. Određeni su najpovoljniji ekstrakcijski uvjeti u kojima je moguće ekstrahirati maksimalnu količinu polifenola, flavonoida i fenolnih kiselina te postići najbolju antioksidativnu aktivnost. Ispitan je i utjecaj liofilizacije na antioksidativnu aktivnost ekstrakata. Postupkom liofilizacije očuvana je količina fenolnih komponenti. Nakon procesa liofilizacije ekstrakata antiradikalna aktivnost raste, a kelirajuća blago pada, što znači da ukupan antioksidativni potencijal ekstrakata ostaje očuvan.

Abstract (english)

The Alfalfa (Medicago sativa L.) is widely used in medicinal purposes. The plant has been reported to have antimicrobial, anti-inflammatory, anticancer and antioxidant effects. These biological activities could be attributed to the presence of both phenolic compounds which act as free radical scavengers and metal chelators. Organic solvents are usually used for the extraction of phenolic compounds from plants which affect adversely on the environment and people. Therefore, the purpose of this diploma thesis was to optimize the extraction conditions of phenol components from Lotus corniculatus L. using aqueous solution of HPβCD. By using the programmes for the statistical design of the experiments it is compared the effect of different process parameters on the extraction efficiency. Some are more favorable extraction conditions in which it is possible to extract the maximum amount of polyphenols, flavonoids and phenolic acids and achieve the best antioxidant activity. The effect of lyophilization is also examined on the antioxidant activity of the extracts. The amount of phenolic components is preserved by freeze drying process. After lyophilization process extracts antiradical activity is growing, and chelating gently fall, which means that the total antioxidant potential of extracts remains preserved.

• Keywords: Medicago sativa L.; ciklodekstrini; polifenoli; antioksidativna aktivnost; liofilizacija
• Keywords (english): Medicago sativa L.; cyclodextrins; polyphenols; antioxidative activity; lyophilization
• Language: croatian
• URN:NBN: urn:nbn:hr:163:522369
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-01-18 16:36:23