Validacija videodenzitometrijske metode za određivanje kvercetina i kemferola u biljnoj vrsti Lotus corniculatus L.

master's thesis

• Title (english): Validation of videodensitometric method for determination of quercetin and kaempherol in Lotus corniculatus L.
• Author: Ivana Kosier
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić (predsjednik povjerenstva)
• Committee member: Suzana Inić (član povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2016-09-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Lotus corniculatus L. široko je rasprostranjena biljka iz porodice Fabaceae bogata polifenolima od kojih su najčešći oni koji za aglikon imaju flavonole kvercetin i kemferol. U ovome radu validirana je videodenzitometrijska metoda za određivanje kvercetina i kemferola iz ekstrakata biljke pripremljenih pri različitim ekstrakcijskim uvjetima. Na temelju istraživanja može se zaključiti kako je ova videodenzitometrijska metoda brza, jednostavna i ekološki prihvatljiva. Rezultati pokazuju kako je ekstrakcija kvercetina i kemferola najbolja pri otapalu kod kojega je omjer vode i etanola 50:50. Validacijski parametri ukazuju na potrebu za daljnjim istraživanjima kako bi se metoda optimizirala pogotovo u slučaju kvercetina koji se ovom metodom ne može pouzdano kvantificirati.

Abstract (english)

Lotus corniculatus L. is an ubiquitous plant from the Fabaceae family, rich in polyphenols among which the most abundant are those having flavonols quercetin and kaempherol as aglycones. In this study, we have validated a videodensitometric method for determination of quercetin and kaempherol from plant extracts prepared under different extracting conditions. Based on the research, it is concluded that this videodensitometric method is fast, simple and ecologically acceptable. Results show that quercetin and kaempferol extraction is greatest when the diluent ratio between water and ethanol is 50:50. Validation parameters show that there is a need for further research in order to optimize the method, especially in case of quercetin which cannot be reliably quantified by this method.

• Keywords: kvercetin; kemferol; Lotus corniculatus; videodenzitometrija
• Keywords (english): quercetin; kaempferol; Lotus corniculatus; videodensitometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:987490
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-09-14 12:49:39