Utjecaj predanalitike na pretrage u likvoru

master's thesis

• Title (english): The influence of pre-analytics on CSF analysis
• Author: Zrinka Drenšek
• Mentor: Dunja Rogić (mentor)
• Committee member: Dunja Rogić (predsjednik povjerenstva)
• Committee member: Olga Gornik (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2017-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

Likvor kao sastavni dio SŢS-a ima veliku važnost u dijagnostici različitih neuroloških bolesti. Zbog izostanka preporučenog protokola o TAT-u za hitne pretrage likvora i utjecaja predanalitike, svrha ovog rada bila je proučiti utjecaj vremena proteklog od uzorkovanja na rezultate citoloških pretraga te ispitati kakav je utjecaj prisutnih staničnih elemenata na rezultate biokemijskih pretraga u likvoru Analiza broja stanica izvršena je u 3 vremenska intervala, ručnim brojanjem u Fuchs-Rosenthalovoj komorici i uz korištenje automatiziranog hematološkog brojača Sysmex XE 5000 unutar 60 minuta od prijema te u Fuchs-Rosenthalovoj komorici nakon 61-90 minuta i 91-120 minuta od prijema. Biokemijske pretrage učinjene su odmah po prijemu i unutar 91-120 minuta u nativnom, necentrifugiranom uzorku i u bistrom supernatantu dobivenom nakon uklanjanja stanica centrifugiranjem Mjerene su koncentracije ukupnih proteina, glukoze i laktata na automatiziranom analizatoru Cobas 6000 c501.Upotrebom statističkog programa MedCalc nije dokazana statistički značajna razlika između broja stanica dobivenih zlatnim standardom u komorici i analizatora Sysmex XE 5000. Analizom je dokazano da se nalaz stanica u intervalu 91-120 minuta promijenio u odnosu na vrijednosti unutar preporučenih 60 minuta što je najviše vidljivo na ukupnom broju stanica i broju velikih limfocita čiji se broj smanjio. Uočeno je da prisutnost stanica u uzorku ima utjecaj na koncentraciju glukoze i laktata dok se koncentracija ukupnih proteina nije mijenjala u promijenjenim uvjetima mjerenja. Ovim radom je dokazano kako se vremenski period za izdavanje nalaza stanica u likvoru može produljiti do 90 minuta te da je za određivanje koncentracije glukoze i laktata uzorak likvora potrebno što prije centifugirati.

Abstract (english)

Cerebrospinal fluid has a very important role in diagnosing various neurologic diseases. Since there are no official protocols concerning the turnaround time (TAT) for emergency tests of CSF and also no information on pre-analytical influence on test results, the main objective of this Thesis was to evaluate the influence of CSF time sampling on cytological results combined with influence of cell elements on biochemistry test results.The cell numbers were analyzed according to three different time intervals. After a 60-minute interval, the cells were counted in the Fuchs-Rosenthal counting chamber and with Sysmex XE 5000 Analyser, whereas only the Fuchs-Rosenthal counting chamber was used for measurements after 61-90 and after 91-120 minute time intervals. The biochemistry tests were conducted immediately upon the receipt of the samples and within the time interval of 91-120 minutes, both in a native sample without performing a centrifugation and in the supernatant without the cells after centrifugation. Glucose, lactate and total proteins were measured with Cobas 6000 c501 Analyser. The implementation of the MedCalc statistical software did not show any statistically relevant differences between cell numbers as counted in the chamber and those measured by the Sysmex XE 5000 Analyser. However, the tests showed that the number of cells counted after the interval of 91-120 minutes is smaller than the number of cells counted after the 60-minute interval, which is clearly visible both in total number of cells and in the number of big lymphocytes. It has been observed that the presence of cells in the sample has influence on the concentration levels of glucose and lactate, whereas the concentration levels of total proteins does not change in different measuring conditions. This study proved that the time of TAT for CSF can be prolonged up to 90 minutes, but when biochemistry test are done, the sample should be centrifuged.

• Keywords: likvor; predanalitika; središnji živčani sustav
• Keywords (english): cerebrospinal fluid; pre-analytics; central nervous sistem
• Language: croatian
• URN:NBN: urn:nbn:hr:163:960192
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije (magistar/magistra medicinske biokemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-16 14:41:00