Utjecaj procesnih parametara visokotlačne homogenizacije i mikrofluidizacije na svojstva i stabilnost nanoemulzija

master's thesis

• Title (english): The effect of process conditions of high pressure homogenization and microfluidization on properties and stability of nanoemulsions
• Author: Anđela Popović
• Mentor: Jasmina Lovrić (mentor)
• Committee member: Jasmina Lovrić (predsjednik povjerenstva)
• Committee member: Anita Hafner (član povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2017-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Nanoemulzije zbog svojih su svojstava zanimljive kao formulacije namijenjene oftalmičkoj
primjeni. Mogu biti izrađene niskoenergetskim i visokoenergetskim metodama. Za potrebe ovog
rada korištene su dvije visokoenergetske metode, visokotlačna homogenizacija i mikrofluidizacija.
Nanoemulzije su pripremljene tako da se najprije izradila primarna emulzija, koja je zatim prošla
kroz jedan, tri i pet ciklusa visokotlačne homogenizacije, odnosno mikrofluidizacije. Veličina
kapljica, PDI i zeta-potencijal određeni su nakon izrade i dva tjedna nakon izrade kako bi se mogla
procijeniti stabilnost nanoemulzija. Dobivene nanoemulzije bile su mliječno bijele boje i nisu
pokazale odvajanje faza tijekom dva tjedna. Općenito su pri većem tlaku i pri većem broju ciklusa
izmjerene najmanje veličine kapljica, dok je najmanja veličina kapljica izmjerena za nanoemulziju
izrađenu nakon pet ciklusa mikrofluidizacije pri tlaku od 1300 bara. PDI nijednom nije bio manji
od 0,1. Zeta-potencijal bio je približno sličan kod svakog uređaja, pri svakom tlaku i broju ciklusa.
Bitno je naglasiti da su izmjerene vrijednosti (~4 mV) daleko od očekivanih vrijednosti (~30 mV).
Nijedna formulacija nije pokazala željena svojstva.

Abstract (english)

Nanoemulsions are interesting as pharmaceutical formulations for ophthalmic use because of their
specific properties. They can be made with low-energy and high-energy methods. High-pressure
homogenization and microfluidization were two high-energy methods used in this research.
Nanoemulsions were prepared as follows: firstly, the primary emulsion was made, which was then
passed through one, three and five cycles of high-pressure homogenization or microfluidization.
Droplet size, PDI and zeta-potential were measured one day after preparation and two weeks after
preparation to assess the stability of nanoemulsions. Prepared nanoemulsions were milky white
and phase separation did not occur in two weeks. In general, using higher pressure and greater
number of cycles resulted with smaller droplet size, with the smallest droplet size measured for
nanoemulsion produced after five microfluidization cycles at 1300 bar. PDI was never less than
0.1. The zeta-potential was approximately the same in nanoemulsions prepared using both devices,
regardless of pressure and number of cycles. It is important to note that the measured values (~ 4
mV) are far from the expected values (~ 30 mV). None of the formulations showed the desired
properties.

• Keywords: nanoemulzije; kitozan; visokotlačna homogenizacija; mikrofluidizacija; oftalmička primjena; mikroemulzije
• Keywords (english): nanoemulsions; chitosan; high-pressure homogenization; microfluidization; ophatlmic application; microemulsions
• Language: croatian
• URN:NBN: urn:nbn:hr:163:580671
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-17 16:51:19