Procjena barijernih značajki animalnih rožnica nakon pohrane u komercijalno dostupnim medijima

master's thesis

• Title (english): Barrier properties of the animal cornea after storage in commercially available mediums
• Author: Sara Ugrinić
• Mentor: Ivan Pepić (mentor)
• Committee member: Ivan Pepić (predsjednik povjerenstva)
• Committee member: Anita Hafner (član povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2017-12-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

S obzirom da je epitel rožnice glavna barijera prijenosu djelatnih tvari preko rožnice, očuvanje
epitela rožnice važno je za provođenje uspješnih ex vivo ispitivanja permeabilnosti.
U ovom diplomskom radu ispitan je utjecaj pohrane animalne rožnice u komercijalno dostupnim
medijima za transport i pohranu rožnica na očuvanje barijernih svojstava rožnice. Barijerna
svojstva rožnica procijenjena su određivanjem transepitelnog električnog otpora. Transepitelni
električni otpor izoliranih animalnih rožnica određen je u vertikalnim protočnim difuzijskim
ćelijama.
Pohranom rožnica u CorneaPrepII® mediju tijekom 24 sata, barijerna svojstva rožnica ostaju
očuvana. Pohranom rožnica u CorneaMax® mediju tijekom 120 sati, barijerna svojstva rožnica se
mijenjaju u ovisnosti o vremenu; nakon 24 sata čuvanja u mediju barijerna svojstva rožnice nisu
bila narušena, dok nakon 120 sati u mediju dolazi do narušavanja barijernih svojstava rožnice.

Abstract (english)

Given that the corneal epithelium is the main barrier to the transfer of the active substances
through the cornea, preservation of the epithelim is important to conduct ex vivo permeability tests.
The aim of this diploma thesis was to examine the effect of the storage of animal cornea in
commercially available mediums for transport and storage on the barrier properties of the cornea.
Barrier properties of the cornea were estimated by measuring transepithelial electric resistance.
Transepithelial electric resistance tests of the isolated animal corneas were performed in vertical
difusion cells, using epithelial voltage clamp.
By storing the corneas in the CorneaPrepII® meduim for 24 hours, the barrier properties of the
corneas remain preserved. By storing the corneas in the CorneaMax® medium for 120 hours, the
barrier properties change depending of the time of storage; after 24 hours of storage in the
medium, the barrier properties of the corneas remain preserved, while after 120 hours of storage
barrier properties were disrupted.

• Keywords: kultura tkiva; transepitelni električni otpor; vertikalne difuzijske ćelije
• Keywords (english): organ culture; transepithelial electric resistance; vertical difusion cells
• Language: croatian
• URN:NBN: urn:nbn:hr:163:950964
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-01-25 13:29:51