Evaluation and selection of optimal solvent and solvent combinations in thin-Iayer chromatography of flavonoids and of phenolic acids of Zizyphus jujuba MilI.

Scientific paper - Original scientific paper

• Author: Marica Medić-Šarić
• Author: Željan Maleš
• Author: Gordana Stanić
• Author: Slavko Šarić
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medicinal chemistry)
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

A series of simple mathematical techniques for the evaluation of
solvents and solvent combinations in thin-layer chromatography
have been investigated. The classification has been carried out using
the numerical taxonomy techniques and information content
derived from Shannon's equation. The methods have been applied
to an RF data set of flavonoids and phenolic acids identified in the
methanolic extract of Zizyphus jujuba MilI. The most suitable chromatographic systems for the separation and future isolation of flavonoids and phenolic acids from Zizyphus jujuba Mill. are : ethyl
acetate : formic acid : acetic acid : water, 100 : 11 : 11 : 27, and
ethyl acetate : formic acid : water, 8 : 1 : 1.

• Keywords (english): thin-layer chromatography; flavonoids; phenolic acids; solvents; Zizyphus jujuba Mill.
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 69, no. 3, pp. 1265-1274
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• URN:NBN: urn:nbn:hr:163:781611
• Publication: 1996-11-01
• Document URL: https://hrcak.srce.hr/177144
• Type of resource: Text
• Access conditions: Open access
• Created on: 2018-01-31 14:30:03