Comparative phytochemical and antimicrobial investigations of Hypericum perforatum L. subsp. perforatum and H. perforatum subsp. angustifolium (DC.) Gaudin

Scientific paper - Original scientific paper

• Title (croatian): Usporedna fitokemijska i antimikrobna istraživanja širokolisne i uskolisne rupičaste pljuskavice [Hypericum perforatum L. subsp. perforatum et H. perforatum subsp. angustifolium (DC.) Gaudin]
• Author: Željan Maleš
• Author: Adelheid H. Brantner
• Author: Katarina Sović
• Author: Kroata Hazler Pilepić
• Author: Miško Plazibat
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical botany)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

The aerial parts of H. perforatum subsp. perforatum and H. perforatum subsp. angustifolium were investigated for their chemical composition and antimicrobial activity. Spectrophotometric analysis indicated that H. perforatum subsp. perforatum is richer in flavonoids and tannins than the other subspecies. HPLC analysis confirmed the higher yield of flavonoids in H. perforatum subsp. perforatum and gave also a higher content of phenolic acids. H. perforatum subsp. angustifolium contained more hypericin. The presence of rutin is proven only in H. perforatum subsp. perforatum. The antimicrobial activity of the extracts of both subspecies was evaluated based on the inhibition zone diameters using the hole-plate diffusion method. The MeOH extracts, the dichloromethane and petroleum ether fractions were effective against Staphylococcus aureus, S. epidermidis, Enterococcus faecalis and Bacillus subtilis. The results indicated that H. perforatum subsp. angustifolium showed a stronger antimicrobial effect than the other subspecies.

Abstract (croatian)

Istražen je kemijski sastav i antimikrobna aktivnost nadzemnih dijelova širokolisne i uskolisne rupičaste pljuskavice [H. perforatum L. subsp. perforatum et H. perforatum subsp. angustifolium (DC.) Gaudin].
Spektrofotometrijskom analizom utvrđena je veća količina flavonoida i trjeslovina u svojti H. perforatum subsp. perforatum. Metodom tekućinske kromatografije visoke moći razlučivanja (HPLC) dokazano je da širokolisna rupičasta pljuskavica sadrži veću količinu flavonoida i fenolnih kiselina, dok je veći sadržaj hipericina utvrđen u uskolisnoj rupičastoj pljuskavici. Prisutnost rutina dokazana je samo u svojti H. perforatum subsp. perforatum.
Antimikrobna aktivnost ekstrakata istraživanih svojti ispitana je metodom difuzije. Metanolni ekstrakti, diklormetanske i petroleterske frakcije pokazali su učinak na sljedeće mikroorganizme: Staphylococcus aureus, S. epidermidis, Enterococcus faecalis i Bacillus subtilis. Svojta H. perforatum subsp. angustifolium pokazala je veći antimikrobni učinak.

• Keywords (english): H. perforatum subsp. Perforatum; H. perforatum subsp. Angustifolium; flavonoids; phenolic acids; hypericin; tannins; HPLC analysis; antimicrobial activity
• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review
• Publication version: Published version
• Journal title: Acta Pharmaceutica
• Numbering: vol. 56, no. 3, pp. 359-367
• p-ISSN: 1330-0075
• e-ISSN: 1846-9558
• URN:NBN: urn:nbn:hr:163:721748
• Publication: 2006-09-01
• Document URL: https://hrcak.srce.hr/4546
• Type of resource: Text
• Access conditions: Open access
• Created on: 2018-02-01 09:02:11