Promjena glikozilacije alfa1-kiselog glikoproteina - utjecaj na konstantu vezanja odabranih lijekova

master's thesis

• Title (english): Change in glycosylation of alpha1-acid glycoprotein - effect on the binding constants of selected drugs
• Author: Hrvoje Curiš
• Mentor: Mario Gabričević (mentor)
• Committee member: Mario Gabričević
• Committee member: Erim Bešić
• Committee member: Tin Weitner
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of general and inorganic chemistry); Zagreb
• Defense date and country: 2015-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U ovom radu ispitivan je efekt promjene konstante vezanja imatiniba, dipiridamola, morfina, imipramina i propranolola na α1-kiseli glikoprotein uslijed promjene u uzorku njegove glikozilacije. Tehnika korištena u ispitivanju bila je fluorescencijska spektrofotometrija. Rezultati ispitivanja ukazuju na to da desijalinizacija α1-kiselog glikoproteina uzrokuje promjenu u
konstantama kemijske ravnoteže između odabranih lijekova i α1-kiselog glikoproteina. Kod imatiniba, imipramina i propranolola desijalinizacija α1-kiselog glikoproteina će uzrokovati smanjenje konstante kemijske ravnoteže između lijekova i α1-kiselog glikoproteina dok će kod dipiridamola i morfina efekt biti suprotan i nakon desijalinizacije α1-kiselog glikoproteina oni će
se jače vezati za desijaliniziranu formu α1-kiselog glikoproteina.

Abstract (english)

In this work an effect of change in glycosylation pattern of α1-acid glycoprotein on the binding constants of imatinib, dipyridamole, morphine, imipramine and propranolol was examined by spectrofluorometric measurements. Results indicate that there is an effect of desialylation of α1-acid glycoprotein on binding constants of selected drugs. Desialylation of α1-acid glycoprotein will reduce the binding constant for imatinib, imipramine and propranolol. On the other hand, dipyridamole and morphine will bind with a higher binding constant to α1-acid glycoprotein after its desialylation.

• Keywords: α1-kiseli glikoprotein; glikozilacija; desijalinizacija; imatinib; dipiridamol; morfin; imipramin; propranolol
• Keywords (english): α1-acid glycoprotein; glycosylation; desialylation; imatinib; dipyridamole; morphine; imipramine; propranolol
• Language: croatian
• URN:NBN: urn:nbn:hr:163:391772
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-01-20 12:15:24