Kvalitativna i kvantitativna analiza flavonoida vrste Laurus nobilis L. (Lauraceae) s područja Dalmacije

master's thesis

• Title (english): Qualitative and quantitative analysis of flavonoids of Laurus nobilis L. (Laureaceae) from the Dalmatian region
• Author: Matea Perica
• Mentor: Renata Jurišić Grubešić (mentor)
• Committee member: Renata Jurišić Grubešić (predsjednik povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Committee member: Živka Juričić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2017-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovoga diplomskog rada provedena je kvalitativna i kvantitativna analiza flavonoida vrste Laurus nobilis L. (Lauraceae) s područja Dalmacije (populacije: Brač, Konavle, Korčula, Lastovo, Mljet, Peljašac i Šipan). Primjenom tankoslojne kromatografije potvrđena je prisutnost kvercetina, rutina, izokvercitrina, kvercitrina i naringina u svim ispitanim metanolnim ekstraktima lista lovora. Kvantitativna analiza flavonoida provedena je spektrofotometrijski. Sadržaj analiziranih flavonoida lovora iznosio je od 0,33% (Pelješac) do 0,62% (Lastovo). Provedena kvalitativna i kvantitatvina analiza predstavlja prilog znanstvenom istraživanju vrste Laurus nobilis L. i upotpunjuje dosadašnje spoznaje o fitoterapijskom potencijalu lovora, posebice u odnosu na sadržaj bioaktivnih flavonoidnih tvari.

Abstract (english)

In this work, qualitative and quantitative analysis of flavonoids of Laurus nobilis L. (Lauraceae) from Dalmatian region was carried out. The thin layer chromatography revealed the presence of quercetin, rutin, isoquercitrin, quercitrin and naringin in all analyzed methanolic extracts of bay leaves (populations: Brač, Konavle, Korčula, Lastovo, Mljet, Pelješac, and Šipan). Quantitative analysis of flavonoids was carried out using the spectrophotometric method. The contents of analyzed flavonoids from laurel leaves varied from 0.33% (Pelješac) to 0.62% (Lastovo). Conducted qualitative and quantitative analysis is a contribution to the scientific study of species Laurus nobilis L. and completes the existing knowledge about phytotherapeutic potential of laurel, especially in relation to the content of bioactive flavonoid substances.

• Keywords: Laurus nobilis L.; flavonoidi; kvalitativna analiza; kvantitativna analiza; UV-Vis spektrofotometrija; tankoslojna kromatografija
• Keywords (english): Laurus nobilis L.; flavonoids; qualitative analysis; quantitative analysis; UV-Vis spectrophotometry; thin layer chromatography
• Language: croatian
• URN:NBN: urn:nbn:hr:163:098775
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-11-28 16:03:39