Mogućnosti primjene HSS-GC-FID tehnike za analizu lakohlapljivih polarnih sastavnica u vlažnim maramicama

master's thesis

• Title (english): Possible application of the HSS-GC-FID technique for the analysis of polar volatile compounds in wet wipes
• Author: Tea Tipurić
• Mentor: Miranda Sertić (mentor)
• Committee member: Miranda Sertić (predsjednik povjerenstva)
• Committee member: Biljana Nigović (član povjerenstva)
• Committee member: Ivana Perković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2018-05-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Brojni kozmetički oblici danas su dostupni u obliku vlažnih maramica pakiranih u plastične omote. Vlažne maramice za jednokratnu uporabu koriste se za njegu dječje kože, pranje ruku, intimnu higijenu, uklanjanje šminke, čišćenje u kućanstvu i dr., a većina se ubraja u kozmetičke proizvode. Samim time, zakonom su regulirane samo neke od sastavnica, dok za ostale većinom nema informacija o količini i potencijalnoj štetnosti na zdravlje.
U ovom radu istražuje se mogućnost primjene metode headspace plinske kromatografije s plamenoionizacijskim detektorom za identifikaciju i kvantitativno određivanje pet odabranih lakohlapljivih sastavnica u maramicama: metanol, etanol, aceton, izopropanol i etil-acetat. Njihova prisutnost u maramicama je dokazana usporedbom vremena zadržavanja analita u uzorku i u standardnoj otopini. Zbog slabe ponovljivosti rezultata mjerenja metoda se nije pokazala pouzdanom za određivanje sadržaja, i daljnja istraživanja treba usmjeriti ka otkrivanju potencijalnih grešaka u analizi, dodatnoj optimizaciji uvjeta ili razvoju sasvim nove metode.
Mjerenja provedena u sklopu ovoga rada otvaraju raspravu o problematici analize sastava čvrstog uzorka bez prethodne ekstrakcije analita i nameću razmatranje uvođenja dodatnih koraka u analizu, što bi mogao biti predmet nekih budućih istraživanja. Kao jedan od navedenih dodatnih koraka posebno se ističe moguća priprema uzorka metodom mikroekstrakcije na čvrstoj fazi (SPME), što svakako ostavlja prostora za istraživanja koja slijede.

Abstract (english)

Numerous cosmetic products are available today in the form of wet wipes packed in plastic wraps. Single-use wet wipes are used for baby skin care, hand washing, intimate hygiene, makeup remover, household cleaning, and most of which are listed as cosmetic products. Consequently, only some of the constituents are regulated by law, while for the rest, there is no information on quantity and potential health hazard.
This paper investigats the possibility of using a previously developed HSS-GC-FID method to determine the presence and content of five selected analytes in wipes: methanol, ethanol, acetone, isopropanol and ethyl acetate. Their presence in wipes was demonstrated by comparing retention times of analytes in the sample and in the standard solution. Due to the poor reproducibility of measurement results, the method did not show any reliability for the determination of content, and further research should focus on detecting potential errors in analysis, additional optimization of conditions, or development of a completely new method.
Measurements carried out within this paper open the discussion on the problem of solid sample composition analysis without previous analytical extraction and require consideration of the introduction of additional steps in the analysis, which could be the subject of some future research. As one of the above mentioned steps, the possible preparation of the sample by the solid phase microextraction method (SPME) is particularly emphasized, which leaves space for further research.

• Keywords: vlažne maramice; HSS-GC-FID; čvrsti uzorak
• Keywords (english): wet wipes; HSS-GC-FID; solid sample
• Language: croatian
• URN:NBN: urn:nbn:hr:163:545629
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-01-16 14:17:22