In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama keratinocita

master's thesis

• Title (english): In vitro biocompatibility study of liposomes differing in (phospho)lipid composition with the keratinocyte cells
• Author: Antonija Katanec
• Mentor: Željka Vanić (mentor)
• Committee member: Željka Vanić (predsjednik povjerenstva)
• Committee member: Anita Hafner (član povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2019-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Različite vrste liposoma, konvencionalni, deformabilni, propilenglikol i kationski liposomi s
uklopljenim azitromicinom, pripremljeni su film metodom. Ekstruzijom kroz polikarbonske
membrane, formulacije liposoma su homogenizirane (indeks polidisperznosti < 0,43), a srednji
promjeri značajno smanjeni (< 250 nm). Fosfolipidni sastav liposoma imao je utjecaja na
površinski naboj liposoma. Konvencionalni, deformabilni i propilenglikol liposomi imali su zeta
potencijale od -48 do -44 mV, dok je zeta potencijal kationskih liposoma iznosio + 62 mV. In vitro
ispitivanja biokompatibilnosti liposoma s uklopljenim azitromicinom provedena na HaCaT
stanicama procijenjena su pomoću MTT testa, mjerenjem metaboličke aktivnosti keratinocita 24
sata nakon inkubacije liposoma s HaCaT stanicama. Uklapanjem u liposome citotoksičnost
azitromicina je smanjena. Fosfolipidni sastav i prisustvo suotapala u liposomima su imali utjecaja
na biokompatibilnost. Propilenglikol je pokazao povoljan učinak na stanice keratinocita, dok su
natrijev deoksikolat i DODAB pokazali potencijalnu citotoksičnost, ali tek pri izuzetno visokim
koncentracijama lipida. Imajući u vidu koncentracije azitromicina u liposomima s kojima je
postignut odgovarajući antibakterijski učinak, svi ispitivani tipovi liposoma su bili biokompatibilni
s HaCaT stanicama.

Abstract (english)

Conventional, deformable, propylene glycol and cationic liposomes encapsulating
azithromycin were prepared by the film hydration method. Extrusion of the liposomal
dispersions through the polycarbonate membranes resulted with liposomes of more
homogenous size distributions with a polydispersity indexes < 0,43 nm and a significantly
reduced mean diameters (< 250 nm). The (phospo)lipid composition affected the surface
charge of the liposomes. Zeta potential values for the conventional, deformable and propylene
glycol liposomes ranged from -48 mV to -44 mV, while the cationic liposomes were shown to
have a positive zeta potential of +62 mV. In vitro biocompatibilities of the different types of
liposomes containing azithromycin with the human HaCaT cells were evaluated 24 h after the
incubation of the cells with the liposomes using the cellular metabolic activity (MTT assay).
The encapsulation of azithromycin in liposomes reduced the cytotoxicity of the drug, whereas
the (phospo)lipid composition and the presence of the surfactant and cosolvent affected the
biocompatibility. Propylene glycol was well tolerated by the keratinocytes in comparison to
sodium deoxycholate and DODAB, where potential cytotoxicities were observed. All types of
the liposomes were biocompatible with the keratinocytes even at the very high concentrations
of azithromycin tested.

• Keywords: liposomi; azitromicin; elastičnost; površinski naboj; propilenglikol; biokompatibilnost; keratinociti
• Keywords (english): liposomes; azithromycin; elasticity; propylene glycol; surface charge; biocompatibility; keratinocytes
• Language: croatian
• URN:NBN: urn:nbn:hr:163:440457
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-21 16:23:59