In vitro ispitivanja biokompatibilnosti liposoma različitog (fosfo)lipidnog sastava sa stanicama fibroblasta

master's thesis

• Title (english): In vitro biocompatibility study of liposomes differing in (phospho)lipid composition with the fibroblast cells
• Author: Ida Kocić
• Mentor: Željka Vanić (mentor)
• Committee member: Željka Vanić (predsjednik povjerenstva)
• Committee member: Anita Hafner (član povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2019-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Svrha ovog rada bila je ispitati biokompatibilnost/citotoksičnost različitih tipova liposoma s azitromicinom na MJ90hTERT fibroblastima. Konvencionalni, deformabilni, propilenglikol i kationski liposomi s azitromicinom međusobno su se razlikovali po (fosfo)lipidnom sastavu i prisustvu suotapala (propilenglikol). Liposomi su pripravljeni tzv. film metodom, a primjenom ekstruzije postignuta je prikladna veličina liposoma za topikalnu primjenu. Sastav liposoma utjecao je na fizikalna svojstva liposoma, posebice naboj na površini i uspješnost uklapanja azitromicina. Provedena in vitro ispitivanja citotoksičnosti pokazala su biokompatibilnost svih ispitivanih liposoma s fibroblastima u koncentracijama azitromicina s kojima se postiže adekvatan antimikrobni učinak liposomskih formulacija. Usporedba različitih tipova liposoma pokazala je najbolju kompatibilnost propilenglikol liposoma, a najmanju kationskih liposoma koja je posljedica prisutnosti kationskog lipida (DODAB-a) u fosfolipidnom dvosloju.

Abstract (english)

The aim of this study was to investigate the biocompatibility/cytotoxicity of the different types of azithromycin-loaded liposomes on the MJ90hTERT fibroblasts. Conventional, deformable, propylene glycol and cationic liposomes encapsulating azithromycin, and differing in (phospho)lipid composition and the presence of co-solvent (propylene glycol), were prepared using the film hydration method. The suitable size of the liposomes for topical application was achieved by their extrusion through the membranes of 400 and 200 nm. The composition of liposomes has proven to influence the physical properties of liposomes, particularly the surface charge and the entrapment efficiency of azithromycin. All the azithromycin-loaded liposomes were biocompatible with the fibroblasts at azithromycin concentrations showing appropriate antibacterial effect. The comparison of the different types of liposomes demonstrated the highest level of compatibility with the MJ90hTERT cells for propylene glycol liposomes and the lowest for cationic liposomes, due to the presence of the cationic lipid (DODAB) in the (phospho)lipid bilayers.

• Keywords: liposomi; azitromicin; propilenglikol; kationski lipidi; elastičnost; fibroblasti; biokompatibilnost
• Keywords (english): Liposomes; azithromycin; propylene glycol; cationic lipids; elasticity; fibroblasts; biocompatibility
• Language: croatian
• URN:NBN: urn:nbn:hr:163:353408
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-21 16:49:49