Citotoksičnost okratoksina A za ljudske stanice pluća i jetre

master's thesis

• Title (english): Cytotoxic effects of ochratoxin A in human lung and liver cells
• Author: Roko Pecotić
• Mentor: Maja Šegvić Klarić (mentor)
• Committee member: Maja Šegvić Klarić (predsjednik povjerenstva)
• Committee member: Dubravka Vitali Čepo (član povjerenstva)
• Committee member: Nevenka Kopjar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of microbiology); Zagreb
• Defense date and country: 2019-06-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Okratoksin A (OTA) je sekundarni metabolit plijesni roda Aspergillus i Penicillium koji je toksičan za
životinje i ljude. Pri kroničnoj izloženosti može dovesti do nefrotoksičnih, imunotoksičnih, hepatotoksičnih,
neurotoksičnih učinaka i do nastanak tumora.
Unos OTA hranom predstavlja najznačajniji rizik za zdravlje koji je ujedno i najviše izučavan. Unos OTA
inhalacijom nije toliko proučen, postoji tek nekoliko istraživanja koja ukazuju na značajnu opasnost zbog
unosa OTA respiratornim putem tijekom profesionalne izloženosti u silosima ili mlinu žitarica.
Svrha ovog rada bila je odrediti citotoksične koncentracije i IC50 OTA na ljudskim stanicama adenokarcinom
pluća A549 i hepatocelularnog karcinom HepG2. Te koncentracije određene su primjenom MTS
proliferacijskog testa i mjerenjem apsorbancije uz pomoć spektrofotometra na 490 nm.
Rezultati su pokazali da su citotoksične koncentracije OTA za obje stanične linije iznosile 50 µM, 75 µM,
100 µM, 150 µM i 200 µM, a dobivena IC50 za A549 stanice je iznosila 83,09±1,04 µM, dok je za HepG2
stanice iznosila 75,00±1,04 µM.
Dobiveni rezultat pokazuju da OTA ima citotoksično djelovanje i na plućnim i jetrenim stanicama ljudskog
porjekla pri čemu su A549 i HepG2 stanice gotovo jednako osjetljive na OTA.

Abstract (english)

Ochratoxin A (OTA) is a secondary metabolite produced by filamentous fungi of the genus Aspergillus and
Penicillium. Chronic exposure can lead to nephrotoxicity, immunotoxicity, hepatotoxicity, neurotoxicity and
cancer.
The intake of OTA by food represents the biggest risk for human health and it is by far the most studied.
Although, inhaled intake has not been studied as much, few studies showed significant impact on human
health due to OTA respiratory intake, especially when it comes to people working in grain storage and mills.
The aim of this study was to determine the cytotoxic concentrations and IC50 of OTA in human cell models
A549 and HepG2. Those concentrations were determined by using MTS proliferation assay after which the
absorbance was measured using a spectrophotometer at 490 nm.
Results showed that the cytotoxic concentrations of OTA for both cell models were 50 µM, 75 µM, 100 µM,
150 µM i 200 µM and the measured IC50 for A459cells and HepG2 cells were 83,09±1,04 µM and 75,00±1,04
µM, respectively.
This study shows that OTA is cytotoxic to both A549 and HepG2 cells and both cell lines are equally
susceptible to OTA.

• Keywords: okratoksin A; A549; HepG2; IC50
• Keywords (english): ochratoxin A; A549; HepG2; IC50
• Language: croatian
• URN:NBN: urn:nbn:hr:163:190645
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-28 12:58:49