Effects of aspirin and indomethacin on galectin-3

Scientific paper - Original scientific paper

• Title (croatian): Utjecaj aspirina i indometacina na galektin-3
• Author: Sanja Dabelić
• Author: Mirna Flögel
• Author: Jerka Dumić
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract (english)

Galectin-3, a β-galactoside binding lectin, acts as a strong pro inflammatory signal. Many immunomodulatory drugs affect signaling pathways that comprise transcription factors involved in regulation of galectin-3 gene (LGALS3) expression. The aim of this study was to investigate the effects of non-steroidal anti-inflammatory drugs aspirin and indomethacin on the expression of galectin-3, both on mRNA and protein levels. The human monocytic cell line THP-1 was exposed to various therapeutic concentrations of aspirin and indomethacin for 72 hours. Relative RT-PCR method and the GeneScan analysis software were used for assessing the galectin-3 mRNA level and chemiluminescent-western blot analysis was used for measuring the galectin-3 level. The results showed that galectin-3 is a new target molecule of NSAIDs, which cause reduction of both gene and protein expression of galectin-3, but the intensity and time-course of the changes strongly depend on the kind and concentration of the drugs.

Abstract (croatian)

Galektin-3, lektin koji veže β-galaktozidne jedinice, djeluje kao jak proinflamatorni signal. Mnoge imunomodulatorne tvari utječu na signalne putove i transkripcijske faktore uključene i u regulaciju ekspresije gena za galektin-3 (LGALS3). Cilj je ovoga rada bio ispitati učinke nesteroidnih tvari s imunomodulatornim djelovanjem – aspirina i indometacina – na ekspresiju galektina-3, kako na razini mRNA tako i na razini proteina. Stanice humane stanične linije THP-1 izložene su različitim terapijskim koncentracijama aspirina i indometacina tijekom 72 sata. Količina mRNA za galektin-3 odre|ena je primjenom metode RT-PCR i analize programom GeneScan, a razina galektina-3 kemiluminescentnom western-blot analizom. Rezultati su pokazali da galektin-3 predstavlja novu ciljnu molekulu djelovanja nesteroidnih imunomodulatornih tvari, koje uzrokuju smanjenje ekspresije gena za galektin-3 i samoga proteina, premda jakost promjena i vrijeme njihove pojave jako ovise o vrsti i koncentraciji primijenjene tvari.

• Keywords (english): aspirin; galectin-3; indomethacin; LGALS3
• Language: english
• Language: croatian
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Croatica Chemica Acta
• Numbering: vol. 78, no. 3, pp. 433-440
• p-ISSN: 0011-1643
• e-ISSN: 1334-417X
• URN:NBN: urn:nbn:hr:163:553609
• Publication: 2005
• Document URL: https://hrcak.srce.hr/54
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-04-21 16:33:49