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Scientific paper - Original scientific paper
Changes of metallothionein, copper, zinc, and zinc-dependent enzymes induced by immobilization stress
Croatica Chemica Acta, 69 (1996), 1; 363-370. urn:nbn:hr:163:772074
Barišić, Karmela; Džanić, Emir; Lauc, Gordan; Dumić, Jerka; Žanić-Grubišić, Tihana; Flögel, Mirna

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Barišić, K., Džanić, E., Lauc, G., Dumić, J., Žanić Grubišić, T. & Flögel, M. (1996). Changes of metallothionein, copper, zinc, and zinc-dependent enzymes induced by immobilization stress. Croatica Chemica Acta, 69. (1), 363-370. Retrieved from https://urn.nsk.hr/urn:nbn:hr:163:772074

Barišić, Karmela, et al. "Changes of metallothionein, copper, zinc, and zinc-dependent enzymes induced by immobilization stress." Croatica Chemica Acta, vol. 69, no. 1, 1996, pp. 363-370. https://urn.nsk.hr/urn:nbn:hr:163:772074

Barišić, Karmela, Emir Džanić, Gordan Lauc, Jerka Dumić, Tihana Žanić Grubišić and Mirna Flögel. "Changes of metallothionein, copper, zinc, and zinc-dependent enzymes induced by immobilization stress." Croatica Chemica Acta 69, no. 1 (1996): 363-370. https://urn.nsk.hr/urn:nbn:hr:163:772074

Barišić, K., et al. (1996) 'Changes of metallothionein, copper, zinc, and zinc-dependent enzymes induced by immobilization stress', Croatica Chemica Acta, 69(1), pp. 363-370. Available at: https://urn.nsk.hr/urn:nbn:hr:163:772074 (Accessed 19 April 2024)

Barišić K, Džanić E, Lauc G, Dumić J, Žanić Grubišić T, Flögel M. Changes of metallothionein, copper, zinc, and zinc-dependent enzymes induced by immobilization stress. Croatica Chemica Acta [Internet]. 1996 [cited 2024 April 19];69(1):363-370. Available at: https://urn.nsk.hr/urn:nbn:hr:163:772074

K. Barišić, E. Džanić, G. Lauc, J. Dumić, T. Žanić Grubišić and M. Flögel, "Changes of metallothionein, copper, zinc, and zinc-dependent enzymes induced by immobilization stress", Croatica Chemica Acta, vol. 69, no. 1, pp. 363-370, 1996. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:163:772074. [Accessed: 19 April 2024]

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Metadata
Title (english)Changes of metallothionein, copper, zinc, and zinc-dependent enzymes induced by immobilization stress
AuthorKarmela Barišić
AuthorEmir Džanić
AuthorGordan Lauc
AuthorJerka Dumić
AuthorTihana Žanić-Grubišić
AuthorMirna Flögel
Author's institutionUniversity of Zagreb
Faculty of Pharmacy and Biochemistry
(Department of biochemistry and molecular biology)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Pharmacy
Medical Biochemistry
Abstract (english)
In order to study the effects of stress on copper (Cu), zinc (Zn), Zn- related enzymes, and metallothionein (MT) in liver, we used the immobilization procedure as an experimental model of stress. Acute immobilization stress caused changes of Zn, Cu, and MT concentrations in rat liver. The Zn level in total liver homogenates increased for 39% (p < 0.00011). This increase originated from the Zn concentration in nuclear fraction, suggesting a higher content of Zn-dependent enzymes and large molecular weight proteins. The stress induced increase of alkaline phosphatase (ALP), which is Zn- related metalloenzyme, closely followed the changes in the Zn concentration in liver. However, the activity of another Zn-dependent enzyme, 5'-nucleotidase (5'-NT), was lower after acute stress. The total Cu concentration was about twice higher in the stressed group in comparison with the control group, but no redistribution of Cu within hepatocytes was found. MT concentration was measured in the mitochondrial-lysosomal fraction of liver homogenates. It was found that acute immobilization stress caused about a 100% increase in the MT concentration in liver.
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCroatica Chemica Acta
Numberingvol. 69, no. 1, pp. 363-370
p-ISSN0011-1643
e-ISSN1334-417X
URN:NBNurn:nbn:hr:163:772074
Publication1996
Document URLhttps://hrcak.srce.hr/135968
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2020-04-29 14:08:20