Optimizacija ekstrakcijskih uvjeta za analizu proizvoda namjenjenih ženama u menopauzi

master's thesis

• Title (english): Optimization of extraction conditions for the analyses of dietary supplements used by women in menopause
• Author: Helena Turkalj
• Mentor: Miranda Sertić (mentor)
• Committee member: Miranda Sertić
• Committee member: Biljana Nigović
• Committee member: Sandra Šupraha Goreta
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2015-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Menopauza je vrijeme u životu žene kada njezine mjesečnice prestaju i tijelo prolazi kroz mnoge promjene praćene brojnim neugodnim simptomima. Danas žene ulaze u menopauzu ranije nego nekoliko desetljeća ranije. Kod mnogih žena simptomi menopauze su toliko jaki da ometaju svakodnevne aktivnosti. Zbog toga mnoge žene koriste dodatke prehrani za ublažavanje tih neugodnih simptoma. Sve se više dodataka prehrani pojavljuje na tržištu te stoga raste potreba za kontrolom kvalitete tih proizvoda. Velika je važnost razvoja novih, selektivnih, osjetljivih i točnih analitičkih metoda za analizu aktivnih sastojaka u takvim proizvodima. Cilj ovoga rada bio je optimizirati ekstrakcijske uvjete za analizu fitoestrogena i njihovo kvantitativno određivanje u različitim biljnim materijalima i dodatcima prehrani namijenjenima ženama u menopauzi. Za optimizaciju ekstrakcijskih uvjeta korištena su tri biljna materijala (soja, konopljika i hmelj). Biljni materijal je homogeniziran, a aktivni sastojci su ekstrahirani u etanolu ili acetonitrilu. Otapala su korištena u koncentracijama 100%, 75% i 50%. Također su istražena različita vremena ekstrakcije (15 min, 30 min i 60 min). Ekstrakcija je provedena pri sobnoj temperaturi korištenjem ultrazvučne kupke. Nakon analize uzoraka novom razvijenom i validiranom LC/DAD/MS/MS metodom, korištenjem Agilent 1100 Series LC/MSD Trap System, kao najbolje otapalo za izolaciju flavonoida iz hmelja je odabran 100% etanol pri ekstrakciji u trajanju 15 minuta.

Abstract (english)

Menopause is a time in a women`s life when her periods eventually stop and the body goes through many changes accompanied by numerous unpleasant menopausal symptoms. Today, women enter the menopause earlier than
couple of decades ago. In many women, symptoms of menopause are so strong that they interfere with their daily life
activities. For that reason, many women use dietary supplements for the relief of these uncomfortable symptoms. More
and more dietary supplements for menopausal symptoms appear on the market and therefore there is a growing need for quality assessment of these products. Development of new, selective, sensitive and accurate analytical methods for the analysis of active compounds in such products is very important. The aim of this study was to optimize extraction conditions for the analysis of active phytoestrogenic ingridients and their quantitative determination in various herbal material and dietary supplements. For the optimization of extraction conditions herbal material of 3 species (Glycine max L., Vitex agnus castus L., Humulus lupulus L.) was used. Material was homogenized and the active ingredients were extracted by ethanol and acetonitrile. Extraction solvents used were of 100%, 75% and 50% concentractions. Also, different extraction time, 15 min, 30 min and 60 min, was investigated. Extraction was performed at room temperature using an ultrasonic bath. After the analysis of the samples by newly developed and validated LC/DAD/MS/MS method, using Agilent 1100 Series LC/MSD trap system, best extraction solvent for flavonoids in Humulus lupulus proved to be 100% ethanol at 15 minutes extraction time.

• Keywords: ekstrakcija; menopauza; flavonoidi; tekućinska kromatografija; masena spektrometrija
• Keywords (english): extraction procedure; menopause; flavonoids; liquid chromatography; mass spectrometry
• Language: croatian
• URN:NBN: urn:nbn:hr:163:389462
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2016-02-18 10:54:45