Kako proteinska korona određuje sudbinu nanočestica u biološkom okolišu

Scientific paper - Review paper

• Title (english): How protein coronas determine the fate of engineered nanoparticles in biological environment
• Author: Ivona Capjak
• Author: Sandra Šupraha Goreta
• Author: Darija Domazet Jurašin https://orcid.org/0000-0001-5261-5961
• Author: Ivana Vinković Vrček https://orcid.org/0000-0003-1382-5581
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract (croatian)

Nanomedicina je iznimno napredno medicinsko područje u kojem se iskorištavaju nanočestice za razvoj inovativnih lijekova, medicinskih pomagala i dijagnostičkih postupaka. U in vivo uvjetima ponašanje nanočestica može biti vrlo kompleksno zbog bliskih interakcija s biološkim molekulama. Zbog međudjelovanja nanočestica i proteina u biološkim tekućinama nastaje dinamička proteinska korona koja obavija nanočestice i tvori novo sučelje između nanočestica i okoliša u kojem se one nalaze (krv, stanice, tkiva). Ta međudjelovanja ovise o biološkim, kemijskim i fizikalnim svojstvima samih nanočestica i proteina, ali i samog biološkog okoliša. U ovom preglednom radu dan je prikaz glavnih karakteristika koji određuju proteinsku koronu te opis njezine dinamičke prirode. Prikazane su najvažnije analitičke metode za istraživanje proteinske korone te primjeri sastava proteinske korone za najčešće korištene vrste nanočestica u biomedicini. Takvo je znanje nužno za dizajn i razvoj učinkovitih i sigurnih nanomedicinskih proizvoda.

Abstract (english)

Nanomedicine is a booming medical field that utilises nanoparticles (NPs) for the development of medicines, medical devices, and diagnostic tools. The behaviour of NPs in vivo may be quite complex due to their interactions with biological molecules. These interactions in biological fluids result in NPs being enveloped by dynamic protein coronas, which serve as an interface between NPs and their environment (blood, cell, tissue). How will the corona interact with this environment will depend on the biological, chemical, and physical properties of NPs, the properties of the proteins that make the corona, as well as the biological environment. This review summarises the main characteristics of protein corona and describes its dynamic nature. It also presents the most common analytical methods to study the corona, including examples of protein corona composition for the most common NPs used in biomedicine. This knowledge is necessary to design NPs that will create a corona with a desired efficiency and safety in clinical use.

• Keywords (croatian): nano-bio interakcije; nanomedicina; meka korona; tvrda korona
• Keywords (english): hard corona; nano-bio interface; nanomedicine; soft corona
• Language: croatian
• Language: english
• Publication type: Scientific paper - Review paper
• Publication status: Published
• Peer review: Peer review - international
• Publication version: Published version
• Journal title: Arhiv za higijenu rada i toksikologiju
• Numbering: vol. 68, no. 4, p. 253
• p-ISSN: 0004-1254
• e-ISSN: 1848-6312
• DOI: https://doi.org/10.1515/aiht-2017-68-3054
• URN:NBN: urn:nbn:hr:163:567635
• Publication: 2017
• Document URL: https://hrcak.srce.hr/191058
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-05-14 15:50:24