Protective effect of flavanone - naringenin on high glucose-induced hepatotoxicity to Hep G2 cells

Scientific paper - Original scientific paper

• Author: Roberta Petlevski
• Author: T. Knežević
• Author: M. Ris
• Author: A. Filipašić
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology)
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract (english)

Naringenin is a flavanone, a type of flavonoid that is considered to have a bioactive
effect on human health as antioxidant, free-radical scavenger, anti-inflammatory,
carbohydrate metabolism promoter, and immune system modulator. This study
was initiated to determine in which of the examined concentrations at the range
1-1000 μM of the naringenin has the protective effect on high glucose-induced toxicity
in Hep G2 cells. The treatment of Hep G2 cells with 30 mM glucose significantly
increased the levels of hepatic functional markers such as AST, ALT, LDH and GGT,
decreased cell viability (MTT test), and decreased the activity ofhepatic antioxidative
enzyme: glutathione peroxidase (GPx), when compared with norma! glucose level
(p<0.05).
We showed, that naringenin at concentration 1 to 100 μmol/L had hepatoprotective
effect on Hep G2 cells under hyperglycemic condition, but at a concentration
of 1000 μmol/L this effect is lost and naringenin showed toxic effect.

• Language: english
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - domestic
• Publication version: Published version
• Journal title: Farmaceutski glasnik
• Numbering: vol. 73, no. 2, pp. 85-97
• p-ISSN: 0014-8202
• URN:NBN: urn:nbn:hr:163:659506
• Publication: 2017
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-06-17 07:30:35