Uspostavljanje sustava tankoslojno kromatografske analize za alprazolam, diazepam, oksazepam i metadon

Scientific paper - Original scientific paper

• Author: Ljubica Tomašek
• Author: Irena Žuntar
• Author: Jasenka Đirlić
• Author: Gordan Mršić
• Author's institution: University of Zagreb; Faculty of Pharmacy and Biochemistry
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract (english)

Thin-layer chromatography is a quick, simple and economic
analytical method for separating different analytes. Hence the purpose
of this work was to validate thin-layer chromatography as an analytical
procedure allowing simultaneous determination of the benzodiazepines
alprazolam, diazepam, oxazepam and the opioid methadone.
Validation of testing quality was performed using two TLC systems: TA
system involving methanol and ammonium mixed in a 100:1.5 ratio and Te
system with dichloromethane and methanol mixed in a 9:1 ratio.
In the process of validating the testing quality, selectivity needs to be
tested and limit of detection must be set for each analyte. For selectivity
testing, standard analyte solutions and certain auxiliary substances were
prepared (matrix), so we could monitor the influence of the matrix on
analyte separation.
Limit of detection was established by preparing a set of dilutions of basic
standard solutions; after chromatogram visualisation, the lowest concentration
for detecting an analyte was set. Limits of detection are: alprazolam
0.80 μg/mL for TA system and 0.53 μg/mL for Te system; diazepam 3.14
μg/mL for TA and Te systems; oxazepam 3.53 μg/mL for TA system and
5.30 μg/mL for Te system, and methadone 6.09 μg/mL for TA system and
9 .14 μg/mL for Te system.
Based on the validation results, we were able to confirm that TLC systems
TA and Te are appropriate for identification of benzodiazepines like alprazolam,
diazepam, oxazepam and of methadone and that auxiliary substances
have no effect on the identification process.

• Keywords (croatian): alprazolam; diazepam; oksazepam; metadon; tankoslojna kromatografija
• Language: croatian
• Publication type: Scientific paper - Original scientific paper
• Publication status: Published
• Peer review: Peer review - domestic
• Publication version: Published version
• Journal title: Farmaceutski glasnik
• Numbering: vol. 65, no. 7-8, pp. 407-415
• p-ISSN: 0014-8202
• URN:NBN: urn:nbn:hr:163:404463
• Publication: 2009
• Type of resource: Text
• Access conditions: Open access
• Created on: 2020-10-07 11:14:30