Isolation of bioactive metabolites from the red alga Sphaerococcus coronopifolius

master's thesis

• Author: Anamarija Tomić
• Mentor: Marijana Zovko Končić (mentor)
• Committee member: Marijana Zovko Končić
• Committee member: Lidija Bach-Rojecky
• Committee member: Jasna Jablan
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacognosy); Zagreb
• Defense date and country: 2015-12-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Kada govorimo o izolaciji bioaktivnih metabolita i komponenti za različite medicinske svrhe, morski organizmi su godinama nedovoljno istraživani u odnosu na većinu kopnenih biljaka. U proteklih nekoliko desetljeća otkad su znanstvenici počeli više istraživati morski okoliš, otkrili su bogatstvo morskih metabolita za široki spektar primjene. Crvena alga Sphaerococcus coronopifolius je morska biljka koja je i ranije često istraživana zbog svojeg kemijskog sastava. Mnogi od njenih izoliranih spojeva, koji spadaju u skupinu bromoditerpena, su pokazali antitumorski i antimikrobni učinak. Ta otkrića su dobra motivacija za znanstvenike da nastave istraživati ovu crvenu algu. Cilj ovog diplomskog rada je bio izolirati i identificirati bioaktivne komponente crvene alge S. coronopifolius. Izolacija bioaktivnih komponenti S. coronopifolius počela je vakuum tekućinskom kromatografijom na početnom organskom ekstraktu, što je rezultiralo većim brojem frakcija. Prva od spomenutih frakcija je identificirana kao allo-aromadendren. Četvrta frakcija je podvrgnuta HPLC analizi, što je rezultiralo izolacijom dviju čistih bioaktivnih komponenti identificiranih kao bromosferol i sferokocenol A.

Abstract (english)

For many years marine organisms have been underinvestigated when compared to most terrestrial plants regarding the isolation of their metabolites and their evaluation for different medical purposes. In last couple of decades that scientists turned their attention on the marine environment, they disovered wealth of marine metabolites for a wide array of applications. The red alga Sphaerococcus coronopifolius is marine plant that has been investigated in the past for its chemistry. Many of its isolated compounds which belong in group of bromoditerpenes showed antitumor and antimicrobial effects. Those discoveries are good motivation for scientists to continue exploring this red alga. The main objective of this diploma thesis was to isolate and identify bioactive compounds of red alga S. coronopifolius. The isolation of the bioactive compounds from S. cornopifolius started with vacuum liquid chromatography of the initial organic extract, which yielded a number of fractions. The first of these fractions was identified as allo-aromadendrene. The fourth fraction was objected to HPLC analyses, which resulted in the isolation of two pure compounds identified as bromosphaerol and sphaerococcenol A.

• Keywords: Sphaerococcus coronopifolius; metaboliti; allo-aromadendren; bromosferol; sferokocenol A
• Keywords (english): Sphaerococcus coronopifolius; metabolites; allo-aromadendrene; bromosphaerol; sphaerococcenol A
• Language: english
• URN:NBN: urn:nbn:hr:163:765070
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-02-25 12:53:37