Mukoadhezivne nanočestice cefiksima

master's thesis

• Title (english): Mucoadhesive nanoparticles of cefixime
• Author: Ana Bajo
• Mentor: Mario Jug (mentor)
• Committee member: Mario Jug (predsjednik povjerenstva)
• Committee member: Željka Vanić (član povjerenstva)
• Committee member: Jasna Jablan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical technology); Zagreb
• Defense date and country: 2017-05-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Mukoadhezivne nanočestice oblik su novog terapijskog sustava s velikim potencijalom za primjenu u lokalnoj i sistemskoj dostavi lijekova. Cilj ovog rada bila je izrada mukoadhezivnih nanočestica cefiksima, njihova fizikalna karakterizacija, optimizacija uvjeta pripreme, ispitivanje fizikalnih svojstava nanočestica nakon postupka liofilizacije te ispitivanje uklapanja lijeka u nanočestice. Za potrebe eksperimentalnog dijela rada proveden je razvoj i validacija HPLC metode. Mukoadhezivne nanočestice cefiksima pripravljene su metodom jednostavne desolvacije te su im određeni veličina, indeks polidisperznosti, zeta potencijal i postotak uklopljenog lijeka. Zeta potencijal čestica kretao se u rasponu od -41,4 do-43,1 mV što ukazuje na stabilnost disperzija nanočestica. Dobiveni rezultati pokazali su da volumen dodane vode utječe na veličinu i indeks polidisperznosti nanočestica. Sadržaj uklopljenog lijeka u nanočestice određen je pomoću tekućinske kromatografije visoke djelotvornosti (HPLC). Iz dobivenih rezultata 1:3 omjer organske i vodene faze dao je najveći postotak uklapanja lijeka u nanočestice. Nakon postupka liofilizacije nanočestica s dva različita krioprotektanta ponovno su određeni veličina, indeks polidisperznosti i zeta potencijal nanočestica. Premda je uklapanje lijeka u nanočestice nisko, ciljana isporuka i bolja bioraspoloživost lijeka omogućuju bolju učinkovitost u odnosu na konvencionalne sustave. U skladu s rezultatima ranijih studija te vlastitim dobivenim eksperimentalnim podacima može se zaključiti da mukoadhezivne nanočestice pokazuju velik potencijal u razvoju novih terapijskih sustava.

Abstract (english)

Mucoadhesive nanoparticles are the form of a new therapeutic system with great potential for local and systemic drug delivery. Compared to conventional therapy, they offer a number of advantages such as targeted drug delivery, prolonged action, drug degradation protection and side effect reduction. The aim of this study was to prepare muchoadhesive nanoparticles of the antibiotic cefixime and perform their physical characterization, optimize the conditions of preparation, to examine the physical properties of nanoparticles after the lyophilization process and to test the drug incorporation. For the experimental part of work, the development and validation of the HPLC method was carried out. The mucoadhesive nanoparticles of cefixime were prepared by a simple desolvation method and their size, index of polydispersity, zeta potencial and percentage of the incorporated drug were determined. The zeta potential of the particles ranged from -41,4 to -43,1 mV, which indicates the stability of nanoparticle dispersion. The results obtained showed that the volume of added water influences the size and index of polydispersity of nanoparticles. The content of the drug in nanoparticles was determined by high performance liquid chromatography (HPLC). From the results obtained 1:3 the ratio of organic and aqueous phases gave the highest percentage of drug incorporation into nanoparticles. After the lyophilization process of nanoparticles with two different cryoprotectans, the size, index of poydispersity and zeta potencial of nanoparticles were again determined. Although drug incorporation into nanoparticles is low, target drug delivery and better bioavilabitity allow better effect than conventional systems. According to the results of earlier studies and our own experimental data, mucoadhesive nanoparticles shows great potential in the development of new therapeutic systems.

• Keywords: mukoadhezivne nanočestice; Gantrez AN; sluznica; cefiksim; uklapanje lijeka
• Keywords (english): mucoadhesive nanoparticles; Gantrez AN; mucus; cefixime; drug incorporation
• Language: croatian
• URN:NBN: urn:nbn:hr:163:574614
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-20 11:55:35