Hipoglikemijski i hepatoprotektivni učinak vrsta roda Globularia na HepG2 stanicama u hiperglikemijskim uvjetima

master's thesis

• Title (english): Hypoglycemic and hepatoprotective effect off genus Globularia in HepG2 cells under hyperglycemic conditions
• Author: Sandra Čuljak
• Mentor: Roberta Petlevski (mentor)
• Committee member: Roberta Petlevski
• Committee member: Željka Vanić
• Committee member: Lovorka Vujić
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2015-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Tretiranje HepG2 stanica 20 mM glukozom uzrokuje hiperglikemijske uvjete koji dovode do oštećenja hepatocita što se očituje porastom jetrenih enzima (ALT i AST). Cilj ovog rada bio je ispitati hepatoprotektivni učinak Globularia spp na aktivnost jetrenih enzima (ALT i AST), ispitati učinak Globularia spp na poboljšanje funkcionalne sposobnosti jetre (sinteza albumina) te učinak na inhibiciju enzima a-glukozidaze, enzima koji katalizira pretvorbu oligosaharida i disaharida u monosaharide. Inhibicijom tog enzima postiže se hipoglikemijski učinak. Aktivnosti enzima izmjereni su u lizatu stanica. Istraživanje je pokazalo statistički značajno povećanje aktivnosti enzima (ALT i AST) u lizatu HepG2 stanica tretirani 20 mM glukozom u odnosu na kontrolnu skupinu (P<0,05). Tretiranje HepG2 stanica Globularia spp (0,5 i I mg/mL) i 20 mM glukozom statistički je značajno smanjilo aktivnost enzima ALT u odnosu na skupinu tretiranu samo 20 mM glukozom. Nije pokazano statistički značajno smanjenje aktivnosti AST nakon tretiranja različitim Globularia spp. Iako je uočen porast u sintezi albumina kod stanica tretiranih Globularia spp taj porast nije statistički značajan. Rezultati ispitivanja učinka Globularia spp na inhibiciju a-glukozidaze pokazali su pozitivan
učinak tj postignuta je statistički značajna inhibicija a-glukozidaze.

Abstract (english)

Treatmet of HepG2 cells with 20 mM glucose causes hyperglycemic conditions , which leads to damage on hepatocytes as evidenced by increased activitiy of liver enzymes (ALT, AST). The aim oft this work is to investigate hepatoprotective effect of Globularia spp on the activity of liver enzymes , effect Globularia spp on the albumin synthesis, and effect Globularia spp on the activitiy of inhibition a-glucosidase. Activities of these enzymes were measured in cell lysates. Treatment of cells with 20 mM glucose significantly (P<0,05) increased activities of liver enzymes compared to the control group. Treatment with 20 mM glucose and Globularia spp significantly {P<0,05) decreased activities of liver enzymes comperd to the group treated only with 20 mM glucose, while treatment with Globularia spp did not decreased activities of liver enzymes. Treatment of cells with Globularia spp did not showed statistically significant (P<0,05) increased albumin synthesis. Treatment of cells with Globularia spp significantly {P<0,05) showed a-glucosidase inhibitory activity.

• Keywords: Globularia spp; kultura stanica hepG2; hiperglikemija; ALT; AST; a-glukozidaza
• Keywords (english): Globularia spp; HepG2 cell culture; hyperglycemic conditions; ALT; AST; a-glucosidase
• Language: croatian
• URN:NBN: urn:nbn:hr:163:243481
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-02-29 15:03:44