Pouzdanost određivanja indeksa hemolize, lipemije i ikterije na koagulacijskim analizatorima

master's thesis

• Title (english): The reliability of the HIL indices assessment on coagulation analysers
• Author: Jozefina Palić
• Mentor: Dunja Rogić (mentor)
• Committee member: Dunja Rogić (predsjednik povjerenstva)
• Committee member: Željka Vogrinc (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of medical biochemistry and haematology); Zagreb
• Defense date and country: 2020-09-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Medical Biochemistry

Abstract

HIL indeksi rezultat su automatske detekcije hemolize, lipemije i ikterije na automatskim analizatorima. Danas su implementirani u sustav većine biokemijskih i koagulacijskih analizatora te su postali zlatni standard u evaluaciji kvalitete uzoraka. HIL indeksi su u sustav koagulacijskih analizatora implementirani tek unazad nekoliko godina te se u praksi rijetko koriste i ne postoji mnogo verifikacijskih studija, iako su nužni zbog postojećeg utjecaja hemolize, lipemije i ikterije na rezultate koagulacijskih pretraga. Cilj ovog rada je stoga bio provjeriti pouzdanost određivanja HIL indeksa na koagulacijskim analizatorima Sysmex CS-5100 i Attelica COAG 360 (Siemens Healthcare, Marburg, Njemačka). Pouzdanost određivanja HIL indeksa provjerena je usporedbom rezultata određivanja kvalitativnih HIL indeksa na analizatoru Sysmex CS-5100 i Attelica COAG 360 s rezultatima određivanja kvantitativnih HIL indeksa na biokemijskom analizatoru Cobas c501 (Roche Diagnostics, Mannheim, Njemačka) i s točnim koncentracijama slobodnog hemoglobina za H indeks, triglicerida za L indeks i ukupnog bilirubina za I indeks u analiziranim uzorcima.
Zaključci ovog rada su da rezultati određivanja HIL indeksa na Sysmex-u CS-5100 i Attelici COAG 360 koreliraju s točnim koncentracijama mjerenih interferenata u uzorku i sa kvantitativnim HIL indeksima na Cobasu c501, da oba analizatora detektiraju prisutnost interferenata već kod vrlo niskih koncentracija te da su HIL indeksi na Sysmex-u CS-5100 i Attelici COAG 360 pouzdani za korištenje u rutinskom radu.

Abstract (english)

HIL indices are the result of automatic detection of haemolysis, lipemia and icterus on automatic analysers. They are determined on most biochemistry and coagulation analysers and have become the golden standard in sample quality evaluation. Only recently HIL indices have been introduced on coagulation analysers, they are rarely used in routine work and there have not been many verification studies regardless of the fact that haemolysis, lipemia and icterus influence coagulation assays. Therefore, the main goal of this study was to verify the reliability of the HIL indices assessment on coagulation analysers Sysmex CS-5100 and Attelica COAG 360 (Siemens Healthcare, Marburg, Germany).The reliability of the HIL indices assessment was examined by comparison of qualitative HIL indices assessment results on coagulation analysers Sysmex CS-5100 and Attelica COAG 360 with the results of the quantitative HIL indices assessment on biochemistry analyser Cobas c501 (Roche Diagnostics, Mannheim, Germany) and with the exact concentrations of free haemoglobin for H index, of triglycerides for L index and of total bilirubin for I index in analysed samples. Main findings of this study are that the HIL indices assessment results on coagulation analysers Sysmex CS-5100 and Attelica COAG 360 are in correlation with measured interference concentrations and with quantitative HIL indices on Cobas c501; further, that both analysers detect very low concentrations of interferences and that the HIL indices on coagulation analysers Sysmex CS-5100 and Attelica COAG 360 are reliable for usage in everyday routine practice.

• Keywords: HIL indeksi; hemoliza; lipemija; ikterija; koagulacijski analizatori
• Keywords (english): HIL indices; haemolysis; lipemia; icterus; coagulation analysers
• Language: croatian
• URN:NBN: urn:nbn:hr:163:793753
• Study programme: Title: Medical biochemistry; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra medicinske biokemije i laboratorijske medicine (magistar/magistra medicinske biokemije i laboratorijske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-30 14:26:41