Promjene N-glikanskih obrazaca imunoglobulina G i glikoproteina plazme uslijed ugradnje želučane premosnice

master's thesis

• Title (english): N-glycosylation changes of plasma proteins and immunoglobulin G in bariatric surgery
• Author: Helena Orehovački
• Mentor: Gordan Lauc (mentor)
• Committee member: Gordan Lauc (predsjednik povjerenstva)
• Committee member: Maja Ortner Hadžiabdić (član povjerenstva)
• Committee member: Lidija Bach-Rojecky (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of biochemistry and molecular biology); Zagreb
• Defense date and country: 2019-09-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Pretilost i dijabetes tipa 2 metabolički su poremećaji i jedni od glavnih javnozdravstvenih problema današnjice. Prati ih stanje kronične upale organizma, međusobno su čvrsto povezani te predstavljaju glavne indikacije za provođenje kirurškog zahvata za liječenje debljine. Navedenom kirurškom intervencijom dolazi do dugotrajne remisije dijabetesa tipa 2 i naglog pada tjelesne mase.
Promjene u ljudskom N-glikomu mogu biti specifične za određena stanja u organizmu te se N-glikane proučava kao potencijalne biljege bolesti, njene progresije i odgovora na terapiju. S ciljem utvrđivanja povezanosti patofizioloških promjena nakon operacije s promjenama N-glikozilacije, u ovom istraživanju analizirani su i uspoređeni N-glikanski profili imunoglobulina G i ukupnih glikoproteina plazme desetorice ispitanika prije ugradnje želučane premosnice i šest mjeseci nakon operativnog zahvata.
Dobiveni rezultati pokazuju određene promjene u N-glikozilacijskom profilu ispitanika između navedenih vremenskih točaka, međutim, opažene razlike nisu dosegle statističku značajnost, najvjerojatnije zbog ograničenog broja ispitanika.
Nadalje, opažene glikozilacijske promjene kreću se u suprotnom smjeru od onih koje se obično uočavaju u upalnim stanjima, što sugerira da je došlo do smanjenja razine upale nakon provedenog operacijskog zahvata. N-glikani imunoglobulina G i glikoproteina plazme time dobivaju ulogu potencijalnih dijagnostičkih i prognostičkih biomarkera za liječenje pretilosti i dijabetesa tipa 2 barijatrijskim zahvatom.

Abstract (english)

Obesity and type 2 diabetes are metabolic disorders which represent significant public healthcare burden globally. Both of them are associated with chronic low-grade inflammation, have a great interdependence and are the main indication for bariatric surgery. This procedure enables long-term type 2 diabetes remission and a significant loss of weight.
Human N-glycome alterations can be specific for certain conditions, hence N-glycans are studied as potential prognostic markers for disease activity, progression and treatment. In order to determine associations between pathophysiological changes after surgery and alterations in N-glycosylation, we analyzed and compared immunoglobulin G and plasma glycoprotein N-glycan profiles from ten subjects, sampled at the baseline and six months after bariatric surgery. There was no statistically significant difference in N-glycome after the mentioned period, probably due to a limited number of examinees.
According to the results, there are several clues that the reduction of inflammation has occurred among the patients. Immunoglobulin G and plasma glycoprotein N-glycans thereby show potential as diagnostic and prognostic biomarkers for bariatric treatment of obesity and type 2 diabetes.

• Keywords: glikozilacija; N-glikani; barijatrija; upala; biomarkeri; HILIC-UPLC
• Keywords (english): glycosylation; N-glycans; bariatric surgery; inflammation; biomarkers; HILIC-UPLC
• Language: croatian
• URN:NBN: urn:nbn:hr:163:474739
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-09-09 12:26:49