Kvalitativna fitokemijska analiza lista i ploda vrste Arbutus unedo L. (Ericaceae) s područja Kvarnera i Dalmacije

master's thesis

• Title (english): Qualitative phytochemical analysis of leaves and fruits of Arbutus unedo L. (Ericaceae) from Kvarner area and Dalmatia
• Author: Monika Perica
• Mentor: Renata Jurišić Grubešić (mentor)
• Committee member: Renata Jurišić Grubešić (predsjednik povjerenstva)
• Committee member: Živka Juričić (član povjerenstva)
• Committee member: Željka Vanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmaceutical analysis); Zagreb
• Defense date and country: 2020-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

U okviru ovoga diplomskog rada provedena je fitokemijska analiza biološki aktivnih tvari listova i plodova vrste Arbutus unedo L. (obična planika, maginja), prikupljenih s otoka Lošinja (lokalitet Mali Lošinj) i Koločepa. Prisutnost polifenolnih spojeva dokazana je kemijskim reakcijama stvaranja obojenih produkata i taloga. Kvalitativna analiza fenolnih kiselina (ferulične, kavene, klorogenske i ružmarinske) te flavonoida (rutin, kvercetin, naringenin) provedena je primjenom tankoslojne kromatografije (TLC). Dokazana je prisutnost flavonoidnih tvari te fenolnih kiselina i/ili iridoida, a sa sigurnošću je detektiran izokvercitrin. Kvalitativna analiza trjeslovina provedena je općim reakcijama stvaranja boje i taloga te je potvrđena prisutnost trjeslovina koje hidroliziraju i kondenziranih trjeslovina u svim ispitanim uzorcima. Kvalitativna analiza triterpena, sterola i triterpenskih kiselina provedena je TLC analizom i potvrđena je njihova prisutnost u svim ispitanim uzorcima. Nije uočena značajna varijabilnost ispitivanih bioaktivnih tvari obične planike s obzirom na različite lokalitete sabiranja biljnog materijala. Provedena fitokemijska karakterizacija predstavlja doprinos dosadašnjem istraživanju roda Arbutus te upotpunjuje znanstvene spoznaje o fitoterapijskom potencijalu maginje.

Abstract (english)

A phytochemical analysis of bioactive compounds of leaves and fruits of Arbutus unedo L. gathered from the islands of Lošinj (population Mali Lošinj) and Koločep has been carried out in this graduate thesis. The presence of polyphenols has been demonstrated by general chemical reactions of developing colored products and precipitates. The qualitative analysis of phenolic acids (ferulic, caffeic, chlorogenic and rosmarinic acid) and flavonoids (rutin, quercetin, naringenin) was perfomed using thin layer chromatography (TLC). The presence of various flavonoid substances, as well as phenolic acids and/or iridoids was noticed, but it has only been confirmed for isoquercitrin. A qualitative analysis of tannins was conducted by general reactions of color and precipitate formation, which confirmed the presence of hydrolysable and condensed tannins in all examined samples. A qualitative analysis of sterols, triterpenic acids and triterpenes was performed by TLC analysis and their presence has been confirmed in all examined samples. Considering the place of collecting, no significant variability in the content of the bioactive substances occurs. The conducted phytochemical characterization represents a contribution to the scientific research of the genus Arbutus, expanding the existing knowledge of biological activity and phytotherapeutic potential of Arbutus unedo L.

• Keywords: Arbutus unedo L.; polifenoli; flavonoidi; trjeslovine; steroli; triterpeni; kvalitativna analiza; TLC
• Keywords (english): Arbutus unedo L. polyphenols; flavonoids; tannins; sterols; triterpenes; qualitative analysis; TLC
• Language: croatian
• URN:NBN: urn:nbn:hr:163:031783
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-02 09:51:50