Lipid droplets in models of Parkinson's and stroke

master's thesis

• Title (croatian): Lipidne kapljice u modelima Parkinsonove bolesti i moždanog udara
• Author: Leo Sabol
• Mentor: Lidija Bach-Rojecky (mentor)
• Committee member: Lidija Bach-Rojecky (predsjednik povjerenstva)
• Committee member: Ana-Marija Domijan (član povjerenstva)
• Committee member: Sandra Šupraha Goreta (član povjerenstva)
• Granter: University of Zagreb; Faculty of Pharmacy and Biochemistry; (Department of pharmacology); Zagreb
• Defense date and country: 2021-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Pharmacy; Pharmacy

Abstract

Parkinson’s disease presents a great challenge in diagnose and treatment, as does stroke. Both require attention to improve these treatments and survivability. Somewhat new and uncharted territory in brain are lipid droplets. They are storage organelles as the pillar of lipid and energy homeostasis, but their function in brain is not yet absolutely defined and it could be of great influence. Goal of this thesis is to positively identify lipid droplets as active participants in neuroinflammation process regarding ischemic stroke and Parkinson’s disease using 6-OHDA models for Parkinson’s and three-vessel occlusion samples for ischemic stroke. Additionally, lipid droplets as participants in neuroinflammation are being identified in thapsigargin treated BV2 cells.

Abstract (english)

Parkinsonova bolest, kao i moždani udar, predstavlja ogromni problem kod dijagnostociranja i liječenja. Oba stanja zahtijevaju trenutnu pažnju za unaprijeđenje liječenja i smanjenje smrtnosti. Jedno od nedovoljno istraženih i novih dijelova tih bolesti su lipidne kapljice. One su, kao lipidni organeli, temeljni stupovi lipidne i energetske homeostaze, no njihova cjelovita uloga u mozgu nije još definirana i mogla bi biti od velikog značenja. Cilj ovog rada je sa sigurnošću identificirati lipidne kapljice kao aktivne sudionike upalnog procesa u mozgu tijekom Parkinsonove bolesti i ishemičnog moždanog udara koristeći modele 6-hidroksidopamina za Parkinsonovu bolest i uzorke sa trožilnom okluzijom za ishemični moždani udar. Također, identifikacija lipidnih kapljica kao aktivnih sudionika upalnih procesa u mozgu vrši se koristeći BV2 stanične culture tretirane s tapsigarginom.

• Keywords: stroke; parkinson’s disease; microglia; rats; mice; inflammation; lipid droplets; cell culture
• Keywords (english): Parkinsonova bolest; moždani udar; mikroglija; miševi; štakori; stanična kultura; lipidne kapljice; upala
• Language: english
• URN:NBN: urn:nbn:hr:163:831460
• Study programme: Title: Pharmacy; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra farmacije (magistar/magistra farmacije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-02-08 13:41:48